Development of Toxic Protein from Entomopathogenic Nematodes Symbiotic Bacteria

Toxic proteins from entomopathogenic nematodes symbiotic bacteria have an obvious poisoning effect on many agricultural pests; entomopathogenic nematode-symbiotic bacterial complex plays a major role in the pathogenicity of insects is symbiotic bacteria. When the nematode enters the insect body, it releases symbiotic bacteria in the blood cavity of the insect, and the symbiotic bacteria reproduce in large numbers and secrete insecticidal substances, killing the insect in a short time. The development of toxic protein from entomopathogenic nematode symbiotic bacteria has good potential for development and application prospects.

Lifeasible is committed to plant protection research, and we aim to provide effective solutions for agricultural and forestry pest prevention and control. In the development of insecticidal proteins from entomopathogenic nematode symbiotic bacteria, we have cloned insecticidal protein genes from symbiotic bacteria for the development of new biopesticides and insect-resistant transgenic crops, which has played an essential role in sustainable agricultural development.

Insecticidal process of toxic proteins from entomopathogenic nematodes symbiotic bacteria

Entomopathogenic nematodes in the infestation stage enter the insect from different orifices of the insect such as anus, stomata, intersegmental membranes, or with the host food, pass through the insect's intestinal wall and enter the body cavity, followed by the release of commensal bacteria carried by the nematodes into the insect's body cavity, these commensal bacteria multiply rapidly in the insect's body and cause septicemia in the host insect, which dies within two days. The nematodes further develop into 4th instar larvae and adults within the host insect, where the nematodes multiply and feed on the symbiotic bacteria within the host insect's carcass while the symbiotic bacteria break down the insect's tissues.

What do we offer?

• Genetic improvement. We genetically improved the entomopathogenic nematode symbiotic bacteria through genetic engineering techniques, such as gene cloning, gene expression regulation, and gene editing, which greatly enhanced their insecticidal protein production and virulence, as well as improved the insecticidal effect and stability of the entomopathogenic nematode symbiotic bacteria insecticidal protein.
• Fermentation culture. We continuously optimize our fermentation technology to enable large-scale cultivation of entomopathogenic nematode symbionts and induce them to produce more insecticidal proteins. In this process, we usually involve medium optimization, fermentation parameter modulation, and strain screening to obtain efficient insecticidal protein yields.
• Extraction and purification. We extract the insecticidal proteins produced by entomopathogenic nematode symbionts from the fermentation broth and go through a series of separation and purification steps such as ultrafiltration, gel chromatography, and high-performance liquid chromatography techniques to remove impurities and obtain high-purity insecticidal proteins.
• Bioactivity identification. This involves testing the virulence activity of different pests, observing the effect of insecticidal proteins on pests, and studying the mechanism of action of insecticidal proteins.

Advantages of our services

Lifeasible has many years of experience in the development of biopesticide. Our solutions play an essential role in promoting sustainable agriculture. If you are interested in us, please feel free to contact us.