Electrochemical Immunosensor for Plant Pathogen Detection

Electrochemical immunosensors are self-contained, integrated systems for specific quantitative or semi-quantitative analysis based on antigen-antibody reactions. Antigens and antibodies are molecular recognition elements in direct contact with the electrochemical sensing element, which converts the signal from the concentration of a chemical substance or class of chemicals into a corresponding electrical signal. Lifeasible can provide you with electrochemical immunosensing systems for detecting plant pathogens, which allow the conversion of biological signals into electrical signals for easy detection and processing. It is fast, accurate, sensitive, and independent of the color and turbidity of the sample, which avoids the disadvantages of radioactive contamination and is non-toxic.

Voltammetry based on enzyme labeling

Enzyme-labeled antibodies bind specifically to antigens or antibodies adsorbed on a solid-phase carrier. Upon dropwise addition of the substrate solution, the substrate may change the hydrogen donor it contains from a colorless reduced form to a colored oxidized form under the action of the enzyme, resulting in a color reaction. Therefore, the color reaction of the substrate can be used to determine the presence or absence of a corresponding immune response. We offer you the electrochemical enzyme-linked immunoassay (ECEIA), which combines an enzyme immunoassay with an electrochemical assay and allows for the detection of plant pathogens with increased sensitivity and selectivity.

• We used ECEIA to detect the Cucumber mosaic virus(CMV).
• We use gold nanoparticles as markers to amplify the analytical signal and significantly increase the sensitivity of the immunoassay. This allowed us to detect Pantoea stewartii sbusp. Stewartii (PSS) in maize.
• We achieved specific detection of rice panicle blight, rice leaf streak, rice cercospora leaf spot, and rice black spot.

Electrochemical impedance spectroscopy (EIS) method

We adopt a label-free format using self-assembled monolayers (SAMs) as an immobilization method to obtain an ordered monomolecular layer on the electrode surface and to obtain a better efficiency of antibody-antigen interactions.

Figure 1. Impedance measurement of electrochemical systems.

• We can use glassy carbon electrodes as sensors for Prunus necrotic ringspot virus (PNRSV) antibody immobilization by covalently attaching specific proteins to the electrodes to detect PNRSV.
• It also has good sensitivity and selectivity for Plum pox virus (PPV) and can detect PNRSV in plant samples diluted tens of thousands of times.

In addition to the above, Lifeasible offers customized services; please feel free to contact us for information.

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