Eucalyptus is native to Australia and its neighboring islands and is one of the world's top three economic and fast-growing tree species. It has been planted on a certain scale in many regions and has produced great ecological and economic benefits. With the increase in pulp demand, Eucalyptus will further expand the cultivation area to meet the market demand. However, the problems of pests, diseases, and freezing seriously affect the further development of Eucalyptus plantation forests. To increase forest yield, shorten the production period, and promote the development of high-quality plantation forests, the genetic engineering of Eucalyptus has received more and more attention. Establishing an efficient and stable in vitro regeneration system for Eucalyptus is the basis for using transgenic means to improve the resistance and quality of Eucalyptus.
Lifeasible is a comprehensive phytotechnology service company. Based on our advanced plant transformation platform and experienced research team, we aim to provide comprehensive technical support services for Eucalyptus genetic transformation. Through an in-depth exploration of Eucalyptus somatic embryogenesis and regulatory mechanisms, we can reveal the molecular genetic mechanism of Eucalyptus embryogenesis and establish an efficient embryonic cell suspension regeneration system and genetic transformation system, which can effectively solve the bottleneck of Eucalyptus genetic engineering breeding in the low rate of genetic transformation. We can be used in the in vitro propagation, fast propagation, detoxification of histocultured seedlings, and the development of new technology in the field of Eucalyptus embryogenesis. It also has broad application prospects in Eucalyptus in vitro propagation, detoxification of histocultured seedlings, and somatic cell hybridization.
Taking a specific Eucalyptus species as the research object, we studied the effects of culture medium, types of plant growth regulators, and their ratios on the induction of Eucalyptus somatic embryo. We established the Eucalyptus embryo regeneration system, and we set up the Eucalyptus embryonic cell suspension regeneration system through the establishment of the Eucalyptus embryonic suspension cell genetic transformation system, laying the foundation for breeding new Eucalyptus varieties through genetic engineering technology. In establishing the Eucalyptus genetic system, we determined the optimal transformation conditions. We provided the root cancer transformation method and Agrobacterium transformation method according to different Eucalyptus varieties to ensure the success rate of transformation.
We first prepared infested Agrobacterium carrying exogenous genes, then prepared Eucalyptus sprouting stakes as explants, then Eucalyptus sprouting stakes were co-cultured with Agrobacterium carrying exogenous genes for genetic transformation and regeneration, and finally, the generated sprouting strips were subjected to selective rooting culture and transplanting to obtain the proposed transformed plants. In our genetic transformation of Eucalyptus, the sprouting stumps of Eucalyptus seedlings were used as explants and transformed by co-culture with Agrobacterium carrying exogenous genes. The sprouting strips grown were selectively cultured in rooting medium, and the proposed transformed plants were obtained by transplanting after rooting. We provide Eucalyptus genetic transformation technology services, which can obtain positively transformed plants in a short period. It is characterized by short cycle time, simple operation, low cost, and wide applicability. It provides a rapid transgenic method for Eucalyptus molecular breeding and batch transgenic production and accelerates the process of Eucalyptus new product breeding.
Lifeasible is committed to providing advanced technical support and reliable solutions to help researchers advance plant genetic transformation research. We have established several plant genetic transformation platforms that can help plant scientists study gene function and breed superior plant varieties. If you are interested in us, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
