Eucalyptus Transformation

Eucalyptus is native to Australia and its neighboring islands and is one of the world's top three economic and fast-growing tree species. It has been planted on a certain scale in many regions and has produced great ecological and economic benefits. With the increase in pulp demand, Eucalyptus will further expand the cultivation area to meet the market demand. However, the problems of pests, diseases, and freezing seriously affect the further development of Eucalyptus plantation forests. To increase forest yield, shorten the production period, and promote the development of high-quality plantation forests, the genetic engineering of Eucalyptus has received more and more attention. Establishing an efficient and stable in vitro regeneration system for Eucalyptus is the basis for using transgenic means to improve the resistance and quality of Eucalyptus.

Lifeasible is a comprehensive phytotechnology service company. Based on our advanced plant transformation platform and experienced research team, we aim to provide comprehensive technical support services for Eucalyptus genetic transformation. Through an in-depth exploration of Eucalyptus somatic embryogenesis and regulatory mechanisms, we can reveal the molecular genetic mechanism of Eucalyptus embryogenesis and establish an efficient embryonic cell suspension regeneration system and genetic transformation system, which can effectively solve the bottleneck of Eucalyptus genetic engineering breeding in the low rate of genetic transformation. We can be used in the in vitro propagation, fast propagation, detoxification of histocultured seedlings, and the development of new technology in the field of Eucalyptus embryogenesis. It also has broad application prospects in Eucalyptus in vitro propagation, detoxification of histocultured seedlings, and somatic cell hybridization.

What do we offer?

Taking a specific Eucalyptus species as the research object, we studied the effects of culture medium, types of plant growth regulators, and their ratios on the induction of Eucalyptus somatic embryo. We established the Eucalyptus embryo regeneration system, and we set up the Eucalyptus embryonic cell suspension regeneration system through the establishment of the Eucalyptus embryonic suspension cell genetic transformation system, laying the foundation for breeding new Eucalyptus varieties through genetic engineering technology. In establishing the Eucalyptus genetic system, we determined the optimal transformation conditions. We provided the root cancer transformation method and Agrobacterium transformation method according to different Eucalyptus varieties to ensure the success rate of transformation.

We first prepared infested Agrobacterium carrying exogenous genes, then prepared Eucalyptus sprouting stakes as explants, then Eucalyptus sprouting stakes were co-cultured with Agrobacterium carrying exogenous genes for genetic transformation and regeneration, and finally, the generated sprouting strips were subjected to selective rooting culture and transplanting to obtain the proposed transformed plants. In our genetic transformation of Eucalyptus, the sprouting stumps of Eucalyptus seedlings were used as explants and transformed by co-culture with Agrobacterium carrying exogenous genes. The sprouting strips grown were selectively cultured in rooting medium, and the proposed transformed plants were obtained by transplanting after rooting. We provide Eucalyptus genetic transformation technology services, which can obtain positively transformed plants in a short period. It is characterized by short cycle time, simple operation, low cost, and wide applicability. It provides a rapid transgenic method for Eucalyptus molecular breeding and batch transgenic production and accelerates the process of Eucalyptus new product breeding.

Our research objectives

• Frost resistance research. With the expansion of the Eucalyptus planting area, low-temperature freezing is the main influence factor for the introduction of Eucalyptus, the expansion of cultivation, and the rapid growth and yield of Eucalyptus. Therefore, the goal of breeding Eucalyptus for cold tolerance is to develop superior varieties with strong cold tolerance. Now, the cold-resistant genes are mainly fish cold-resistant protein genes, ice nucleation genes from bacteria, etc. The research on disease and pest resistance of Eucalyptus.
• Resistance to pests and diseases. There are more than 200 kinds of pests and diseases in Eucalyptus, among which, cyprinosis is the primary disease that jeopardizes the growth of Eucalyptus, and the occurrence and prevalence of cyprinosis in recent years have brought significant economic losses to the Eucalyptus industry. Using the Agrobacterium transformation method, insect and herbicide-resistant transgenic Eucalyptus plants have been successfully obtained.
• Lignin improvement. Eucalyptus is one of the most essential raw materials for the paper industry. Still, the environmental pollution caused by pulp and paper waste liquid is extremely serious, and the source material is the lignin in the raw materials for paper making. We investigated the reasons affecting lignin biosynthesis by examining the genes of relevant enzymes used in genetic engineering for lignin improvement in forest trees and the enzymes involved in the lignin biosynthesis pathway. The research we have provided provides an essential basis for improving the wood properties of paper-making resource plants.

Note to Customers

• We are available all year round to conduct experiments on plant genetic transformation technology services.
• Customers can make personalized requests for project service options and delivery standards, and both parties can negotiate to determine the technical solutions and costs.
• Due to various factors, the cost of experiments may be adjusted from time to time, so please get in touch with us directly for details.

Our service workflow

Lifeasible is committed to providing advanced technical support and reliable solutions to help researchers advance plant genetic transformation research. We have established several plant genetic transformation platforms that can help plant scientists study gene function and breed superior plant varieties. If you are interested in us, please feel free to contact us.