Exosome Lipidomics

The entire life cycle of exosomes, from early endosome development to mature multi cyst formation to exosome transport and uptake, requires the involvement of large amounts of lipids such as cholesterol, phospholipids, and sphingolipids, which are vital substances for exosome phospholipid bilayer formation. Compared to donor cells, exosomes are more enriched in sphingolipids, cholesterol, and phosphatidylserine. The lipid composition of the membrane structure of exosomes from different sources is selective.

Figure 1. Schematic diagram of lipids in exosomes. (Skotland et al., 2020)

The lipid composition of exosomes is like that of lipid rafts, and exosomes have a higher lipid content than other extracellular vesicles and a higher stability to detergents. Exosomal lipid molecules can interact with receptors such as G proteins and lipid molecules on the recipient cell membrane, which regulates signaling pathways and biological activities within the recipient cell. Exosomal lipidomic analysis can be used to identify novel diagnostic markers and exosome bioactivity analysis. The lipidomic analysis is characterized by high lipid homology, difficult identification, and high sample purity requirements. The exosomal lipidomic analysis also has the general problem of a small sample size, which is challenging to accurately characterize and quantify exosomal lipids. To obtain higher quality exosomal lipidomics data, Lifeasible has developed unique technologies such as exosome isolation and purification to ensure high purity of exosome samples prior to testing.

Our Services

From exosome isolation, lipidomic analysis, and data analysis to assay reporting, Lifeasible provides reliable, rapid, and cost-effective one-stop exosome lipidomics services based on highly stable, reproducible, and sensitive isolation, characterization, identification, and quantitative analysis systems.

Exosomal Lipidomics Services

• Analysis and identification of lipids and their metabolites
• Study of lipid metabolic pathways and networks
• Lipid function and metabolic regulation

Data Analysis

• Data pre-processing
• Lipid classification
• Dendrogram analysis
• Multivariate statistical analysis
• Differential lipid screening
• Differential lipid statistical analysis
• Differential Lipid Hierarchical Clustering Analysis
• Differential Lipid Association Plot

Our exosome lipidomics service provides a better understanding of the importance of lipid-based biomarkers and the role of lipids in vesicle biogenesis and function. Simply send us your samples or purified exosomes if you need them, and we will send you the analytical data as soon as possible.

Exosomal Lipidomics Services Technical Route

Advantages of Our Services

• High Test Quality

High-precision instrumentation is used for testing, which allows you to obtain accurate mass spectra.

• Standardized Platform for Strict Quality Control

Complete process standard operating procedures guidance.

• Short Project Cycle Time

Through process optimization, the whole project cycle time is shorter than the market.

• After-sales Service is Available

The after-sales service team has rich experience in lipidomics experiments and can better understand customer needs.

If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.