The main goal of exosomal metabolomics technology is to study the relationship between metabolites and physiological and pathological changes in the body qualitatively and quantitatively. Regarding research methods, metabolomics mainly consists of targeted and untargeted metabolomics. Non-targeted metabolomics is full metabolomics for a certain physiological state, and the advantage of identifying differential metabolites briefly is its comprehensiveness. Targeted metabolomics, on the other hand, is for specific metabolites, with standards as a reference, for relative or absolute quantitative analysis, with better sensitivity and accuracy. With the increased resolution and scanning speed, high-resolution mass spectrometry has been applied to metabolomics of different diseases.
Figure 1. Untargeted metabolomics analysis of EVs molecular cargo. (Dudzik et al., 2021)
Plant exosome metabolomics, which focuses on analyzing the changes of metabolites at different developmental stages or under specific treatments, reveals the metabolic mechanisms. Plant exosome metabolomics helps to reveal information about the overall metabolite dynamics in plant samples and is a powerful tool for analyzing plant exosome metabolic pathways and regulatory mechanisms under specific conditions.
Lifeasible relies on a mature experimental system, one-stop solution service features, and selects high-efficiency liquid phase separation as well as high-resolution mass spectrometry detection system with high resolution and good stability. The standard process, strict quality control, and rich data analysis fully assist your scientific research.
Assays are performed using hydrophilic interaction chromatography, identifying a wide range of known metabolites from exosomal samples, identifying positive or negative pattern analytes by retention time matching and accurate mass.
The detection of metabolites from samples with different treatments or physiological states allows obtaining of differential metabolites between states, thus exploring the mechanisms of differences and finding biomarkers that can be used in the fields of plant resistance, plant nutrition, and medicinal value evaluation. For biological replicates, n ≥ 10 is recommended for replicate sample analysis.
Lifeasible can provide efficient and accurate plant exosome metabolomics analysis services, specifically for primary and secondary metabolism analysis. We customize our services to your specific research requirements and ensure a comprehensive analysis service of the highest precision.
If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.
Reference
