Exosome RNA Analysis

Exosomes contain protein components and RNA components, such as micro-RNA, miRNA, long-stranded non-coding RNA (lncRNA), mRNA, and circular RNA (circRNA). These RNAs carried by exosomes are collectively referred to as exosome-derived RNAs and have complete sequence structure and biological activity. Lifeasible, with its rich experience in RNA projects and leading biomarkers, has launched a system solution for exosomal RNA research, which will assist researchers in carrying out in-depth analysis on exosomal mRNA, miRNA, lncRNA, etc. It is mainly applied in the study of growth and development regulation of plants and animals, disease and stress resistance regulation, biological traits, and mutation regulation; in the research of diseases, it can study the mechanism of disease occurrence and regulation, search for biomarkers, target drug research.

Figure 1. Analysis of RNA from cells and exosomes. (Jenjaroenpun et al., 2013)

Exosomal RNA Analysis Services

1. Exosomal mRNA Sequencing

Lifeasible's mRNA sequencing services are differentiated for different sample types and can detect not only routine samples but also degraded samples and micro samples. Customers have the flexibility to choose according to their samples.

2. Exosome miRNA Sequencing

Our professionals use proven techniques to enrich exosomes, perform RNA extraction, miRNA-seq library construction, and high-throughput sequencing of exosomal fractions in samples, which can obtain millions of miRNA sequences at one time, enabling rapid identification of known and unknown miRNAs and their expression differences in different tissues, different developmental stages, and different disease states.

3. Exosomal circRNA Sequencing

By enriching circRNA through our technology platform, based on high-throughput sequencing, we can obtain a large amount of circRNA information and predict the structure and target targets of cirRNA through bioinformatic analysis. The information available includes:

• Predictive identification of circRNAs
• Annotation of linear genes where circRNAs are located
• Localization of circRNAs on the genome
• Quantitative analysis of circRNA expression
• Analysis of expression differences between samples
4. Exosomal lncRNA Sequencing

Lifeasible provides exosomal long-chain non-coding RNA sequencing and analysis of all exosomal-derived LncRNA and mRNA in samples. The information available for analysis includes:

• lncRNA, mRNA, circRNA identification
• lncRNA, mRNA, circRNA quantitative analysis
• lncRNA, mRNA, circRNA differential expression analysis
• lncRNA, mRNA, circRNA expression/differential gene clustering

Customers only need to provide cell supernatant and exosome RNA. Lifeasible will complete the whole service process from sample preparation, sample isolation, sample enrichment, library preparation, and up-sequencing to data analysis.

Our Advantages

• Professional exosome research technology service provider with rich experience in exosome RNA projects.
• Professional assay platform to meet different analysis needs of exosomal RNA.
• Multiple sets of exosomal RNA research solutions to meet different research needs of exosomal mRNA, lncRNA, miRNA, and circRNA.
• With a strong bioinformatics team, we can meet customers' requirements for various types of in-depth analysis of data.

If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.