Fatty acids are a class of aliphatic carboxylic acid compounds containing long-chain hydrocarbons, usually in the form of esters, found in various lipid fractions such as neutral fats, phospholipids, and glycolipids. Fatty acids can be classified into various types according to different classification criteria; for example, according to the length of the carbon chain, they can be classified into short-chain fatty acids (SCFA), medium-chain fatty acids (MCFA), and long-chain fatty acids (LCFA). Lifeasible follows the cutting-edge direction of fatty acid quantification and constantly updates its testing technology, aiming to provide more sensitive and accurate fatty acid quantification analysis services.

Importance of Fatty Acids Quantification

• Improvement in the quality of oilseed crops. Quantitative analysis of the fatty acid composition of oilseed crops helps select and breed varieties with high oleic acid content, thereby improving the quality of vegetable oils.
• Identification of edible oil adulteration. Based on the fatty acid composition of oils and fats, adulteration of edible oils can be recognized.
• Development of oil and fat resources. The type, content, and proportion of fatty acids determine the quality and utilization of fats and oils, and fatty acids are quantitatively analyzed to discover and replace new oil and fat resources.
• Nutritional value assessment of agricultural products. Determination of the content of major fatty acids in edible beans can provide data support for the evaluation of nutritional value and comprehensive development and utilization of edible beans.

Our Service for Fatty Acids Quantification

Gas chromatography and gas chromatography-mass spectrometry (GC-MS), both well known for their great sensitivity and accuracy, are used in real-world applications. To increase the precision and dependability of our findings, we constantly refine our testing procedures. Based on sample characteristics and analytical requirements, we will choose the best fatty acid quantification method for various customer projects. For instance, we would suggest GC-MS for complicated samples that need accurate fatty acid species and content identification. In contrast, we might choose TLC or HPLC techniques for samples that need quick screening.

Thin layer chromatography (TLC)

This assay is characterized by a simple and rapid qualitative analysis, achieved by spotting the sample on a chromatographic plate and using the difference in partition coefficients of the different fatty acids in the stationary and mobile phases.TLC is suitable for preliminary screening and rough analysis of fatty acids.

Gas chromatography (GC)

GC is one of the most widely used methods for fatty acid analysis, utilizing the difference in retention time of different fatty acids in the stationary phase to achieve separation and detection.GC is characterized by high sensitivity, high resolution, and rapid analysis, and it is suitable for the quantitative analysis of fatty acids in medium and long chains.

High performance liquid chromatography (HPLC)

Unlike GC, HPLC uses a liquid as the mobile phase and is suitable for analyzing fatty acids that are more polar or thermally unstable.HPLC provides higher separation efficiency and more accurate quantitative results.

Gas chromatography-mass spectrometry (GC-MS)

GC-MS combines the separation capability of GC with the identification capability of MS, enabling qualitative and quantitative analysis of fatty acids. This method is particularly suitable for identifying and quantifying fatty acids in complex samples as it provides information on the molecular structure.

Sample Request

• Type. Plant and animal tissues (≥0.5 g/sample), microorganisms (≥0.2 g/sample), fermentation broth (≥20 ml/sample).
• Preservation. Liquid nitrogen quick-freeze and store at -80°C.
• Transportation. Dry ice transportation.

Our Research Application of Fatty Acids Quantification

• Compositional studies of medicinal and oleaginous plants.
• Metabolite kinetic analysis.
• Metabolic pathway analysis.
• Lipid metabolism studies.
• Standardized testing of food and edible oils.
• Nutritional and developmental studies.

Highlights of Our Fatty Acids Quantification

• We have established various detection technology platforms, including gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS).
• We can accurately quantify a wide range of short-chain fatty acids, medium- and long-chain free fatty acids, and medium- and long-chain total fatty acids to ensure the accuracy of the results.
• We adopt a strict quality control system to ensure the accuracy of quantitative data.
• We provide a complete and professional after-sales service system to ensure that our customers receive perfect technical support throughout the entire research process.

Fig.2 Our service advantages. (Lifeasible)

Relying on advanced technology and professional services, Lifeasible is committed to providing customers with high-quality solutions for fatty acid quantification. If you are interested, please feel free to contact us.