Genetic Engineering of Insecticidal Proteins

Pests cause substantial yield losses in agricultural systems and pose a significant threat to global food security. Traditional chemical insecticides play a crucial role in pest control. However, concerns about environmental contamination, toxicity to non-target species, and resistance development have prompted the search for alternative solutions. By developing customized novel insecticidal solutions using genetic engineering-related technologies, target pest populations can be controlled efficiently and effectively while minimizing adverse impacts on non-target organisms and the environment.

Lifeasible specializes in life sciences and biotechnology development. With our advanced technology platforms and dedicated research teams, our genetic engineering technology provides a more precise and environmentally friendly control management strategy for pest control.

Our solutions

• Identification of Bt pesticide protein genotypes
• Identification of insecticide protein genes
• Genetic engineering of insect resistance
• Development of insecticidal hormones

What do we offer?

We offer advanced genetic engineering technologies to provide effective pest control solutions. The most important is RNA interference technology. RNAi links altered phenotypes to gene function by inhibiting the expression of target genes, providing an effective guidance strategy for insect control. Currently, we offer RNAi capable of controlling target pest population size by suppressing essential genes to increase insect mortality. In addition, we can rapidly analyze gene functions using dsRNA-mediated RNAi technology.

In addition, we also screen for miRNAs that negatively regulate their autoimmune signaling pathways in pests in the control of target pests, construct engineered strains in fungi through genetic engineering modification, and transport these miRNAs across the borders from fungal cells into infected target pest cells to target the pest signaling pathway or transcriptional expression, which can be effectively used to accelerate the rate of death of target pests.

The Process of RNAi-based genetic engineering for insect resistance

• Target gene selection. Selection of relevant genes for the target pest, usually those critical to the pest's life cycle, physiological processes, growth, and development.
• siRNA design. Design of small interfering RNA (siRNA), composed of exogenous double-stranded RNA fragments that partially match the mRNA sequence of the target gene. When siRNA enters the plant cell, it binds to the mRNA of the target gene. This results in degradation of the target mRNA or blockage of translation, thus inhibiting the expression of the target gene.
• Construction of RNAi vector. The designed siRNA sequence is inserted into an appropriate vector, usually a plant expression vector. This vector also needs to contain some necessary regulatory elements, such as promoters, terminators, etc., to ensure that the siRNA is expressed in plant cells.
• Preparation of transgenic plants. The constructed RNAi vector is introduced into the target plant cells, then the transgenic plant will integrate this RNAi vector and express the siRNA.
• RNAi effect. The transgenic plant produces siRNAs that interfere with the expression of genes in the target pest. When the pest ingests these transgenic plants, the ingested siRNAs enter the pest cells and suppress the expression of the target gene.
• Insect resistance effect. After the pest ingests the siRNA, inhibition of the target gene may lead to abnormal growth and development or death of the pest. This is the anti-insect effect.

Our service workflow

Lifeasible has many years of research and service experience in insecticidal protein development and application. Based on our advanced technology platform, we provide various comprehensive and effective solutions for identifying insecticidal protein genes, genetic engineering of insect resistance, and RNAi-based control in pests. If you are interested in us, please feel free to contact us.