Toxicokinetics of Herbal Medicines

Toxicokinetics is the application of pharmacokinetic principles and methods to quantitatively study the absorption, distribution, metabolism, and excretion processes and characteristics of drugs in the body at toxic doses and thus to investigate the regularity of the occurrence and development of drug toxicity. Lifeasible provides a toxicokinetic analysis service for herbal medicines, establishing concentration-response relationships and concentration-effect relationships. The aim is to reveal the intrinsic link between the systemic exposure achieved and the onset of toxicity under toxicity testing conditions, to compare the value of systemic exposure with that of humans in interpreting toxicological data, and to support the design of protocols for herbal toxicity studies.

• Exposure assessment. Species differences in plasma protein binding, tissue uptake, receptor properties, metabolic characteristics, pharmacological activity, immunogenicity, and toxicological effects of metabolites.
• Toxicokinetic parameters. Kinetic parameters are calculated by measuring sample concentrations at appropriate time points.
• Dosing regimens. Dose, route, choice of animal species, and frequency and periodicity of administration.
• Sample collection. The collection is the frequency required to achieve exposure evaluation.
• Methods

Cytotoxicity test MTT method

We directly observe the toxic response of herbs at the cytological level with the help of cell biology techniques. This includes the effects on the morphological structure of cells and organelles and the effects on the overall cellular activity. The MTT method is a rapid and efficient way to detect the effects of drugs on cell proliferation and toxic responses. This is a microscopic response to the biological basis of the toxicological effects of herbal drugs.

Herbal serum pharmacology method

The toxic effects of herbal drugs can be directly observed in cells cultured with drug-containing serum from experimental animals to elucidate the toxicology from a microscopic perspective. We have initiated this protocol to provide a new option for elucidating the mechanism of herbal toxicity at the cellular-molecular level.

Molecular biology techniques

We apply molecular biology methods to herbal toxicology studies, including determining nucleic acid content in toxic organs, determining drug-metabolizing enzymes and antioxidant enzyme activities in drug-injured hepatocytes, and exploring toxicological genomics and proteomics. This will help you to investigate the mechanisms and nature of herbal toxicity at the genomic level.

• Data statistics and evaluation. Calculate the mean or median and assess the variance.

Lifeasible's toxicokinetic analysis service can be used to describe the systemic exposure of subjects and/or their metabolites in toxicity tests and their dose and time related to the toxic response, to evaluate the toxic response of subjects and/or their metabolites in different animal species, sexes, ages and body states (e.g., gestational state), and to evaluate the selection of animal species and dosing regimens for non-clinical toxicity studies. The rationality of animal species selection and dosing regimens for non-clinical toxicity studies. Please feel free to contact us to improve the predictive value of animal toxicity test results for clinical safety evaluation.

• Toxicity Analysis of Herbal Medicines