Analysis of Protein Modification Expression Profiles in Insects

Compared with western blotting and other techniques, mass spectrometry can more effectively analyze the post-translational modification of proteins, and can complement the conventional Western blot post-translational modification protein identification. Mass spectrometry analysis of protein post-translational modifications generally uses a bottom-up peptide-based approach. However, the bottom-up mass spectrometry method cannot guarantee that the specific post-translational modification of the target protein can be fully identified, because mass spectrometry is identified by multiple peptides within the protein sequence, which greatly reduces the chance of identifying post-translational modified peptides.

Our Services for Analysis of Protein Modification Expression Profiles in Insects

With the improvement of the sensitivity and resolution of mass spectrometry, the method of sample separation and fractionation is further optimized, and the sequencing depth of PTM continues to increase. The expression profiling research of deep sequencing technology on other PTMs constantly refreshes the researchers' existing cognition. All this shows us the great research potential of PTM expression profiling. Lifeasible can provide analysis of protein modification expression profiles in insects to analyze several types of expression profiles of different proteins in different growth, development and metabolic processes of insects.

Based on the property of LC-MS/ MS, we can detect several types of protein modification expression profile in following species.

Our Methods for Analysis of Protein Modification Expression Profiles in Insects

Lifeasible uses LC-MS/ MS to analyze the expression profiles of insect modified proteins at different growth and developmental stages and under different stresses. We provide the list of protein identifications, statistical mapping of modification sites; modification site identification list, GO functional enrichment map; peptide conserved motif analysis list, KEGG pathway map; GO function list, conserved motif analysis; KEGG signaling pathway mapping table and protein interaction network analysis.

Service Flow

Fig 1. Service flow for the analysis protein modification expression profiles in insects.

Applications

• Phosphorylation: signal transduction, cell cycle, growth and development and cancer mechanisms.
• Glycosylation: protein folding, renewal, and immune response, etc.
• Acetylation: regulation of gene expression, apoptosis, cellular metabolism, protein stability, and neurodegenerative pathologies.
• Ubiquitination: cell cycle, apoptosis, transcriptional regulation, DNA repair, and signal transduction.
• Methanation: chromatin structure and transcriptional regulation, etc.
• Malonylation/ malondiylation/ glutarylation/ succinylation: closely related to metabolic processes.

Advantages

• Enriched by CST gold-labeled antibody, with high specificity and reliable results.
• High identification throughput, can detect up to 25,000+ phosphorylation modification sites.

Lifeasible provides services with several types of protein modification expression profiles in some insects to detect various expression profiles of different proteins in the growth development of insects based on LC-MS/ MS to analyze metabolic processes, immune response and other development in the growth of insect. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.