Analysis of Protein Modification Sites in Insects

Protein post-translational modifications (PTMs) occurs when specific positions in the amino acid sequence of a protein are covalently bound to chemical moieties or small molecular weight proteins, resulting in an increase in the molecular weight of a specific sequence compared to the unmodified protein. During the identification of PTMs, proteins are first cleaved into peptides and then analysed by mass spectrometry, which provides information on the molecular mass of a series of peptides.

Our Services for Analysis of Protein Modification Sites in Insects

Mass spectrometry analysis of protein PTMs generally uses a bottom-up peptide-based approach. One strategy to increase the number of modified peptides detected by mass spectrometry is to use PTM affinity reagents for peptide enrichment rather than protein enrichment using PTM affinity reagents, which will reduce the number of enriched unmodified peptides. Lifeasible can provide analysis of insect protein modification sites to detect the specific sites of modifications occur on insect proteins or peptides.

Based on the property of LC-MS/ MS analysis to accurately determine the molecular mass of proteins or peptides, we can detect the following modification types in several species.

Our Methods for Analysis of Protein Modification Sites in Insects

In the process of mass spectrometry analysis of protein PTM, the protein is first cut into peptides by enzymes, and then enters the mass spectrometer for analysis; through mass spectrometry, the relative molecular mass information of a series of peptides is obtained. Post-translationally modified proteins have an increase in the molecular weight of a specific peptide sequence compared to a protein without PTM. For example, phosphorylation modification, so if it is found that the molecular weight of some peptides has just increased by the weight of a phosphate during the mass spectrometry detection process, it can be assumed that the peptide has been phosphorylated and modified, and then through the spectrum of the secondary or multi-level mass spectrometry, the identification of the PTM type and the analysis of the modification site can be realized.

Service Flow

Fig 1. Service flow for the analysis of insect protein modification sites.

Applications

With several types of the insect protein modification sites analysis to detect the specific sites of modifications occur on insect proteins or peptides accurately based on LC-MS/ MS.

Advantages

• Three conventional modification types can be scanned at one time.
• The analysis is not limited to conventional PTM, and personalized targeted modification analysis can be performed according to the determined amino acid position and modification type.

Lifeasible provides services with several types of the insect protein modification sites analysis to detect the specific sites of modifications occur on insect proteins or peptides accurately based on LC-MS/ MS. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.