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Baculovirus Expression System

Baculovirus Expression System

Undefined insect cell culture media components make it difficult to obtain consistent performance. This system is the first baculovirus expression system that includes chemically defined media and protein expression enhancers to provide reliable and trustworthy results. This insect cell expression system also utilizes a non-engineered derivative of Sf9 insect cells that has been adapted for high density suspension growth and insect cell-specific vectors. It can produce three times more protein than existing insect expression platforms. This system improves the consistency of multiple expression runs in less time and accelerats development from small to large scale.

Our Services for Baculovirus Expression System

The Baculovirus Expression System provides convenient virus generation and protein expression cell lines for secreted and intracellular proteins in just two steps, and we predict that using the system will save us approximately 30% of protein expression time and make cell maintenance easier. Lifeasible provides protein expression services with Baculovirus expression system. This expression system is designed to provide consistent cell growth and protein expression, with protein yields far superior to traditional insect platforms. Suspension transfection of bacmid DNA using this system enables efficient baculovirus production without the need for virus amplification. The system also accelerates development from small to large scale. ExpiSf system delivers the same high levels of volumetric yield, making the system excellent for both discovering new therapies and producing them in large quantities.

The conponents of this syetem we provide are listed below.

System Components included in system
Baculovirus expression system
  • Two vials of frozen sf9 Cells
  • 1 L Medium
  • One Bac-to-Bac Vector Kit
  • 4 mL Enhancer
  • 0.5 mL MAX Efficiency DH10Bac Competent Cells
  • 1 mL Fectamine Sf Transfection Reagent
  • 500 mL Opti-MEM I Medium, with phenol red
  • 24 Nalgene Single-Use PETG vented 125-mL Erlenmeyer Flasks

Advantages

  • Consistent results-chemically defined, yeastolate-free, animal origin-free growth medium supports consistent cell growth and protein expression.
  • Streamlined workflow-go from bacmid DNA transfection to protein expression in half the time: only 6-10 days compared to 10-20 days via standard protocols.
  • Scalable from discovery to production-the scalability of the system accelerates development by delivering equivalent cell growth and protein volumetric yield from small to large scale.
  • Higher protein yield-the system demonstrated greater protein expression-delivering more than 3 times protein.

Lifeasible provides protein expression services with baculovirus expression system. We offer higher expressed protein expression, highly consistent results, and more streamlined steps to shorten experiment time and other several advantages by our professional team. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.

Our Services are for Research Use Only. Not for Clinical Use!
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