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Bemisia tabaci is a globally important agricultural pest and one of the most important vectors of many plant viruses. It is extremely omnivorous and invasive and is found in tropical, subtropical, and temperate regions worldwide. It damages plants by feeding on the phloem and transmitting pathogenic viruses, causing significant economic losses worldwide.
Bemisia tabaci has become resistant to chemical insecticides, and new control strategies are needed to stop its continued spread. Lifeasible efficiently delivered nucleic acids and proteins into developing Bemisia tabaci embryos using feeding and microinjection procedures. This leads to new ways to help you study this vital pest of global agriculture by using the CRISPR system to make effective, heritable edits in the genome of Bemisia tabaci progeny.
| Editable genes | Relevant traits exhibited after editing |
| TRP | We provide CRISPR protocols suitable for Bemisia tabaci TRP genes, enabling you to utilize the protocols to induce mutations in Bemisia tabaci to obtain stable mutant phenotypes. Our use of the CRISPR system to repress the expression of the target gene at the nuclear gene level can meet your needs to further utilize induced mutations for the biological control of pests. We have optimized the synthesis primers for the target site of sgRNA in the Bemisia tabaci TRP gene sequence to overcome the off-target effect and can inhibit the expression of the target gene at the nuclear gene level to achieve a durable and stable effect and mutant phenotype. |
If the RNA interference technique is further employed, the expression of the Bemisia tabaci TRP gene is effectively and transiently suppressed by feeding dsRNA. After the TRP gene of Bemisia tabaci was partially silenced, it could not effectively sense the external high-temperature stress, blocked the signaling cascade response, and the expression of heat stress protein in the cell could not be elevated in time, which led to the decrease of heat resistance and the increase of mortality of Bemisia tabaci.
Traditional Bemisia tabaci control strategies make it difficult to meet the needs of agricultural production, and new means of control are urgently needed. Lifeasible's easy-to-use CRISPR gene editing program for Bemisia tabaci will allow researchers to apply the power of reverse genetic approaches to the species to bring new methods of control to this destructive pest. If you are interested in other target genes such as Dnmt, Hsps, etc., feel free to contact us for a proposal.
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