Gene Editing in Bombyx Mori

Bombyx mori is a lepidopteran silk-producing insect that feeds on mulberry leaves. The silkworm passes through four morphologically and physiologically distinct stages of development: egg, larva, pupa, and adult, and different parts of each stage of development and their metabolites have a wide range of medicinal uses. Currently, bombyx mori genome targeted editing technology has been widely used in the functional analysis and identification of unknown endogenous genes in bombyx mori, development of silk gland bioreactors, cocoon silk synthesis and secretion mechanism, sex regulation, resistance breeding and other research fields.

Our Services for Gene Editing in Bombyx Mori

The main types of genome manipulation include targeted mutation of endogenous genes in bombyx mori, genome structural variation, targeted integration and knockout of exogenous genes in bombyx mori genome, etc. Lifeasible provides gene editing services for bombyx mori with zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALEN) and CRISPR/Cas9 to optimize genetic traits of bombyx mori.

Editable genes	Relevant traits exhibited after editing
*BmVNCP*	We can edit the BmVNCP gene in bombyx mori cells or tissues to affect the immune machinery of bombyx mori.
*Bmnsd-Z* *Bmnsd-2*	We can edit the Bmnsd-Z and Bmnsd-2 genes to affect the immune system of bombyx mori, to build a disease resistant bombyx mori strain with excellent traits, and to improve the quality of cocoon silk under the premise of disease resistance.
*EcR*	We can edit the EcR to affect the immune system of bombyx mori and influence the resistance of bombyx mori to viruses.
*SPI51* *Seroin1*	We can edit SPI51 and Seroin1, to affect the process of silk fibrillation or the conformational shift of silk proteins within the silk gland lumen, where the silk protein form changes from a liquid to a solid state. We can edit the silk protein gene make abnormalities in silk properties and other aspects of silk, or only silk glue cocoons can be formed.
*BmSP2*	We can edit BmSP2, resulting in insufficient expression of antimicrobial peptides produced by bombyx mori when threatened by pathogens, thus leading to reduced immune resistance of bombyx mori and greater susceptibility to infestation by pathogenic microorganisms.

Advantages

Edit immune system-related genes that affect the immune response of bombyx mori.
Edit the genes involved in the apoptotic pathway activated by ecdysteroids to affect the role of bombyx mori in disease resistance.
Edit the silk protein gene to affect the silk properties of bombyx mori.
Edit the storage protein-related genes to affect growth and reproduction, metamorphosis development and immune resistance in bombyx mori.

Lifeasible provides gene editing services for bombyx mori by ZFNs, TALEN and CRISPR/Cas9 to affect the silk properties, and growth and reproduction, the development of metamorphosis and immune system of bombyx mori to optimize genetic traits of bombyx mori. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.

Our Services are for Research Use Only. Not for Clinical Use!

Services

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