Gene Editing in Chilo Suppressalis

Chilo suppressalis is a moth of the family Crambidae. It is a serious pest of rice. They bore the stems of their host plants, and therefore are classified as rice stem borers. Almost all plant parts, from twigs, leaves, stems are attacked. Caterpillars can be largely internal feeders, whereas adults are external sap feeders. Heavily attacked plants can show varying symptoms from dead heart, white heads, dwarfing, stunting, rot, abnormal forms, and rosetting. Finally, the whole plant will die. Dead hearts are the most obvious field symptoms.

Our Services for Gene Editing in Chilo Suppressalis

At present, the control strategy of chilo suppressalis is a combination of prevention, avoidance and control. Based on agricultural control, chemical pesticides are applied rationally on the basis of grasping the occurrence period, occurrence amount and damage degree of the pest. But with the emergence of drug resistance, new ways to control these pests are urgently needed. Lifeasible provides gene editing services for chilo suppressalis with zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALEN) and CRISPR/Cas9 to control their reproduction and reduce the harm caused by chilo suppressalis.

Editable genes	Relevant traits exhibited after editing
CsLCP16 CsLCP17	We can provide the knockdown of CsLCP16 and CsLCP17 genes to inhibit the growth and development of the epidermis and head of chilo suppressalis larvae and result in molt failure.
*CsGSTs*	We can edit CsGSTs family genes to affect the role in detoxification metabolism of certain drugs in chilo suppressalis.
*Csebony*	We can knockdown the Csebony cause melanin deposition in chilo suppressalis larvae and pupae as well as adults and regulate epidermal coloration of chilo suppressalis.
*CsCncC* *CsKeap1*	We can edit CsCncC and CsKeap1 to affect chilo suppressalis adaptation to chemical stresses.
*CsABCs*	We can edit CsABCs genes such as CsABCC8, CsABCH1 and CsABCCE1 to affect the translocation metabolism of insecticides in chilo suppressalis and increase their resistance.
*CsMet1* *CsMet2*	We can make the CsMet1 or CsMet2, resulting in a lower number of mature eggs in female moths and affecting the reproductive process of chilo suppressalis.
*CsAK*	We can knockdown the CsAK genes, resulting in abnormal energy metabolism and growth and development in chilo suppressalis.
*CsCYP4M38 CsCYP4M39*	We can edit CsCYP4M38 and CsCYP4M39 to affect the detoxification metabolism of chilo suppressalis, and knockdown of these genes to increase the susceptibility of chilo suppressalis to drug resistance.

Advantage

Edit resistance-related genes affect resistance of chilo suppressalis to pesticides.
Editing certain genes that control body color to regulate epidermal coloration of chilo suppressalis.
Editing growth and development-related genes to affect energy metabolism and growth and developmental processes such as head, and molt in chilo suppressalis.
Editing of reproduction-related genes reduces the reproduction of chilo suppressalis.

Lifeasible offers gene editing services for chilo suppressalis by three means include ZFNs, TALEN and CRISPR/Cas9 to edit some genes to control this organism like reducing the reproduction and the resistance to pesticides, affecting the growth and developmental processes. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.

Reference

Bai, Q.; et al. Spatial and temporal expression analysis of two P450 genes, CYP4M38 and CYP4M39, in Diplodocus chinensis [J]. Zhejiang Agricultural Journal, 2018, 30(04): 521-527.

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