Gene Editing in Danaus Plexippus

Danaus plexippus is a milkweed butterfly in the family Nymphalidae. It is amongst the most familiar of North American butterflies and an iconic pollinator, although it is not an especially effective pollinator of milkweeds. The monarch was the first butterfly to have its genome sequenced. The genome provides researchers insights into migratory behavior, the circadian clock, juvenile hormone pathways, and microRNAs that are differentially expressed between summer and migratory monarchs. More recently, the genetic basis of monarch migration and warning coloration has been described. New genome editing technology is facilitating functional validation of these mutations in whole organisms, including the monarch butterfly (danaus plexippus).

Our Services for Gene Editing in Danaus Plexippus

Danaus plexippus is able to help pollinate plants and has been listed as an endangered species because its population has been greatly reduced by the excessive use of chemical pesticides. Lifeasible provides the gene editing service for danaus plexippus with zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALEN) and CRISPR/Cas9 technology to change the growth and developmentof danaus plexippus.

Our Methods for Gene Editing in Danaus Plexippus

• ZFNs

Lifeasible offers ZFNs to edit danaus plexippus. The target gene of danaus plexippus was selected and gene knockout was successfully achieved by in vitro synthesized mRNA injection, and the knockout rate in cell S phase is up to 90%. Experiments on gene fragment insertion can also be performed. Gene insertion and knockdown are obtained at different stages of the cell cycle with different results. However, there are difficulties in designing effective ZFNs and the cost is high.

• TALEN

Lifeasible provides gene editing services in danaus plexippus through TALEN. We use two methods to knockout gene in danaus plexippus. The first method is knockout gene by transgene after constructing the vector. Another is gene knockout can be performed by injecting mRNA synthesized in vitro, and the knockout rate in danaus plexippus can reach 90%.

• CRISPR/Cas9

Lifeasible provides the technology of CRISPR/Cas9 in danaus plexippus. We can edit the danaus plexippus with gene knock out, tag insertion and gene fragment insertion.

Advantages

• Gene insertion and knockdown in danaus plexippus are obtained differently at different stages of the cell cycle.
• Make the precise insertion of tag into the target site in the species of danaus plexippus.
• The knockout rate in danaus plexippus can reach 90% or more.
• Editing certain genes to affect the growth and development of danaus plexippus.

Lifeasible provides three technology of gene editing in danaus plexippus and later services by our professional team with the advantage of obtained the different results of gene insertion and knockdown in danaus plexippus according to different stages of the cell cycle and high efficiency. If you are interested in our services, please click online inquiry for more detailed information.