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Gryllus bimaculatus belongs to the order Orthoptera, with over 6,000 extant species. Edible insects, especially crickets, are of general interest due to their better nutritional value and high food conversion efficiency. Gryllus bimaculatus is an essential mini-edible insect used for animal feed and human food. It's a typical chirping insect and an emerging model organism of increasing importance.
Genome editing is an effective method for site-specific genetic manipulation in various species. Gryllus bimaculatus, as a model organism, has the advantages of easy reproduction, large egg production, detailed embryonic developmental stage charts, and assembled and annotated genomes. Lifeasible can target knockout and knock-in of target genes in Gryllus bimaculatus using the CRISPR/Cas9 system and also offers knockout services through TALEN. To help you conduct in-depth research in the fields of Gryllus bimaculatus behavior, neurology, physiology, genetics, species formation, and acoustic communication.
| Editable genes | Relevant traits exhibited after editing |
| Tyrosine hydroxylase (TH), yellow-y | The process of cuticle hardening is complex, and insect cuticles are soft and pale when initially synthesized. TH and yellow-y are involved in the insect melanin and catecholamine biosynthesis pathways, and mutations in both genes result in pigmentation defects. This allows us to leverage CRISPR/Cas9 to provide you with a featured animal model for studying cuticle color's key role and protective mechanisms. You can also use Gryllus bimaculatus as a model system for regulating melanin synthesis. |
| Ubx, abd-A | Based on Cas9 mRNA/sgRNA, we can perform knockout operations on this gene to regulate tissue and organ morphogenesis. In addition to targeted sequence deletion, we can also provide targeted sequence knock-in, a highly desirable technique that will expand your ability to understand the function of the genomic region of interest. |
| lac2 | TALEN is an artificial nuclease that induces DNA double-strand breaks into specific target sequences. The gene regulates the tanning of the cuticle after molting in arthropods, and the loss-of-function phenotype of the gene is readily detectable. We can provide TALEN-based knockout services in Gryllus bimaculatus, including determining pure knockout organisms by mutation detection. This approach is not associated with a specific genotype or phenotype. |
To facilitate the production of pure knockout Gryllus bimaculatus individuals through genome editing, Lifeasible offers CRISPR/Cas9 system-based targeted knockout and knock-in services and TALEN knockout services, as well as the necessary RNA interference services. Please feel free to contact us for more details.
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