Gene Editing in Helicoverpa Armigera

Helicoverpa armigera is a species of Lepidoptera in the family Noctuidae, one of the most destructive pests in the world. A combination of molecular genetics, physiology, functional genomics, and behavioral studies has made helicoverpa armigera a model species in Lepidoptera Noctuidae. CRISPR/Cas9 genome editing technology is a convenient and effective method used for performing functional genomic studies. Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALEN) are also means of insect genetic manipulation.

Our Services for Gene Editing in Helicoverpa Armigera

Gene editing technology for helicoverpa armigera has efficient insecticidal effects in controlling pests in the field. Lifeasible provides the gene editing service for helicoverpa armigera with gene editing technology of ZFNs, TALEN, and CRISPR/Cas9include the gene of CTLs and drug tolerance-related genes like ABCA2 to reduce this kind of pests.

Our Methods for Gene Editing in Helicoverpa Armigera

• ZFNs

Lifeasible offers ZFNs to edit helicoverpa armigera by promoting genome-targeted editing of proteins by creating double-strand breaks in DNA at locations you specify. We engineered zinc finger domains to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within helicoverpa armigera complex genomes and alter the genomes of helicoverpa armigera.

• TALEN

Lifeasible provides the technology of TALEN gene editing in helicoverpa armigera to cut specific sequences of DNA. We fusing a TAL effector DNA binding domain with a DNA cutting domain, a nuclease that cuts the DNA strand to cleave specific DNA sequences in helicoverpa armigera.

• CRISPR/Cas9

Lifeasible provides gene editing in helicoverpa armigera through the system of CRISPR/Cas9. We use three methods to edit the helicoverpa armigera with gene knock out, tag insertion and gene fragment insertion.

1. Introduce a plasmid vector to encode Cas9 RNA and sgRNA in vitro;
2. Injecting plasmid sgRNA-related DNA into transgenic helicoverpa armigera;
3. Establish a transgenic model containing Cas9-sgRNA.

Advantages

• By editing certain genes, we could reduce the drug resistance of helicoverpa armigera.
• By editing immune-related genes to affect the natural immune defense system of helicoverpa armigera was reduced, which in turn reduced this type of pests.
• Tracing the protein expression profile of the target gene by inserting the tag at the protein level.

Lifeasible provides gene editing in helicoverpa armigera and later services. With the advantage of precise knock-out of genomic fragments, single-base substitution of genomic DNA, precise insertion of tag into the target site and tracing the protein expression profile of the target gene, if you are interested in our services, please click online inquiry for more detailed information.