Gene Editing in Junonia coenia

Junonia coenia belongs to the family Nymphalidae and has a broad larval diet. Commonly found in cities, suburbs, towns, coastlines, roadsides, fields and meadows, and other open areas. They are sensitive to cold and cannot winter in icy northern areas. The common Junonia coenia is mostly brown with eyespots on the wings. The eyespot pattern found on the wings of butterflies is one of the most striking color patterns in nature, and there is a vast amount of literature on their ecology, evolution, and development.

Our Services for Gene Editing in Junonia coenia

The forewings and hindwings of Junonia coenia are distinct from each other, and their color patterns and morphology serve a wide range of functions in flight, signaling, and protection. A complex collection of genes may be responsible for this phenomenon, and they interact to form the final pattern. Lifeasible offers Junonia coenia gene editing services based on CRISPR/Cas9 technology to help you explore the wing eyespot's regulation modes.

Technology process

• Sample preparation.
• sgRNA preparation. Two single sgRNAs were used to generate two Cas9 cleavage sites on either side of the desired knockout spacer, and then non-homologous end joining was relied upon to close the resulting gaps to generate long deletions in the target motif.
• Embryo microinjection
• Mutant genotyping and trait scoring. Non-CRISPR mutations were excluded when scoring phenotypes.

Lifeasible helps you find the essential genes that act as master regulators of butterfly wing color by providing Junonia coenia gene editing services based on CRISPR/Cas9 technology. Please feel free to contact us to customize more editing services for critical genes.