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Gene Editing in Laodelphax Striatellus

Gene Editing in Laodelphax Striatellus

Gene Editing in Laodelphax Striatellus

Laodelphax striatellus is a species of the genus Lilaphax belonging to the Homoptera Planthopper family. Because the host is a variety of turfgrass and rice, wheat, corn, barnyard grass and other gramineous plants, it is very harmful to agriculture. It is a pest that occurs intermittently in recent years. Its virus-carrying individuals are directly related to the epidemic of rice, wheat and corn virus diseases. Generally, the climate is warm in winter and relatively dry in spring, which is a harbinger of the large occurrence of the first generation of laodelphax striatellus.

Our Services for Gene Editing in Laodelphax Striatellus

The main hazard of laodelphax striatellus is the transmission of viruses. In areas where rice virus diseases are endemic, the purpose of pest control and disease prevention should be ruthless control of laodelphax striatellus in the most susceptible seedling stage and early Honda stage, including agricultural control, biological control and chemical control. Lifeasible provides gene editing services for laodelphax striatellus with zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALEN) and CRISPR/Cas9 to control them.

Editable genes Relevant traits exhibited after editing
LsAKH
LsAKHR
  • We can edit LsAKH and LsAKHR to affect triglyceride content in the laodelphax striatellus, which in turn affects its resistance.
LsOBP4
LsOBP7
  • We can edit LsOBP4 and LsOBP7 to affect the different behavioral responses of the laodelphax striatellus to rice compounds.
LsMETTL3
LsMETTL14
  • We can knockdown the LsMETTL3 and LsMETTL14, affecting the development of the laodelphax striatellus.
LsSFP450s
  • We can knockdown the LsSFP450-2, LsSFP450-8, LsSFP450-14, LsSFP450-12, LsSFP450-4, LsSFP450-5, LsSFP450-10, LsSFP450-7, LsSFP450-15, LsSFP450-9, affecting the detoxification metabolic pathway of laodelphax striatellus, and improving the insecticide resistance of the laodelphax striatellus.

Our Methods for Gene Editing in Laodelphax Striatellus

  • ZFNs

Lifeasible offers ZFNs to edit some genes in laodelphax striatellus. We use ZFNs to obtain the results according to your needs. The editing rate is not less than 90%.

  • TALEN

Lifeasible provides two methods to edit the specific gene in laodelphax striatellus through TALEN. One method is constructing the vector and then transgene to knockout gene. Another is injecting mRNA synthesized in vitro of laodelphax striatellus.

  • CRISPR/Cas9

Lifeasible provides CRISPR/Cas9 to edit the gene in laodelphax striatellus. We use this technology to edit the gene in larvae and adults include the female and male of laodelphax striatellus with high efficiency and mutate multiple genes simultaneously.

Advantages

  • Editing resistance-related genes to affect the detoxification metabolic pathway of laodelphax striatellus and improve the resistance to pesticides.
  • The editing of growth-related genes, affects the growth and development laodelphax striatellus to control this organism.

Lifeasible provides gene editing services for laodelphax striatellus by three means include ZFNs, TALEN and CRISPR/Cas9 to increase the susceptibility to drug resistance and affect the growth and development of this organism to control them. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.

Reference
  1. Zhang, J.H.; et al. Cytochrome P450 Monooxygenases CYP6AY3 and CYP6CW1 Regulate Rice Black-Streaked Dwarf Virus Replication in Laodelphax striatellus (Fallén). Viruses, 2021, 13(8):1576.
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