Gene Editing in Mythimna Separata

Mythimna separata is a moth of the family Noctuidae. It is found in China, Japan, South-east Asia, India, eastern Australia, New Zealand, and some Pacific islands. It is one of the major pests of maize in Asia. The larvae eat leaves, and can eat up all the leaves of crops when they occur, causing serious losses. Because of its flocking, migratory, omnivorous, and gluttonous properties, it has become a nationally important agricultural pest. Larva can be hand-picked and adults can be attracted using pheromones. Regulation of water level in the fields, and removal of alternate hosts from the field are also effective. Selective pesticides and weedicides can also used in the field. New, effective methods are needed to control this organism.

Our Services for Gene Editing in Mythimna Separata

Mythimna separata is a typical seasonal long-distance migratory pest, which mainly damages grain crops such as corn and wheat. Three conventional control methods are available, including biological trapping of adults, physical trapping of adults and pharmaceutical control. And gene editing technology is the most effective method. Lifeasible provides the gene editing services for mythimna separata with zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALEN) and CRISPR/Cas9 to control their reproduction and reduce the harm caused by mythimna separata.

Editable genes	Relevant traits exhibited after editing
*Chitinase*	We can knockout or knockdown the Chitinase like Chi1 and Chi2 to improve the mortality of larvae and provide a new method for mythimna separata control.
*EcR*	We can edit the EcR to regulate molting of mythimna separata, and control mythimna separata.
*CYP4G199*	We can edit the CYP4G199 to affect the mucor epidermal development, reduce the resistance of mythimna separata, thus helping to develop new P450 inhibitors or insecticides.
*OBP6* *OBP11*	We can edit OBP6 and OBP11 to affect the foraging behavior of mythimna separata, and reduce the damage of mythimna separata to crops.
*PBPs*	We can edit the MsepPBP1 and MsepPBP3 to reduce the sensitivity of mythimna separata males to sex pheromone molecules and thus reduce mating and larval birth rates.

Advantages

Editing key genes for growth and development to improve the mortality of larvae of mythimna separata.
Edit genes related to odor proteins, to affect the foraging behavior of mythimna separata.
Editing some drug resistance-related genes to reduce resistance to drug and improve the resistance susceptibility to pesticides of mythimna separata and control this organism.

Lifeasible offers three means include ZFNs, TALEN and CRISPR/Cas9 to edit some genes to control mythimna separata by reducing larval birth rates and drug resistance or altering their foraging behavior. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.

Reference

Liu, Y.; et al. A potential target for Mythimna separata (Walker) control. Arch Insect Biochem Physiol, 2020, 104(1): e21666.

Our Services are for Research Use Only. Not for Clinical Use!