Glycosylation of proteins is one of the common and important post-translational modifications of proteins in organisms. It is a process in which sugars are transferred to proteins under the action of glycosyltransferases and form glycosidic bonds with special amino acid residues on proteins. More than 50% of proteins in cells are modified with sugar chains. N-glycosylation attached to asparagine (Asn) residues is ubiquitous in eukaryotes and is the most well-studied form of glycosylation. Protein N-glycosylation mainly plays a key role in the process of complex multicellular or tissue formation, and participates in life activities such as cell recognition, immune response, and cell differentiation.
Glycosylation regulates the location, function, activity, lifespan and diversity of proteins in tissues and cells, and participates in various important life activities such as cell recognition, differentiation, development, signal transduction, and immune response. In the occurrence and development of various diseases, such as metabolic diseases, immune diseases and infectious diseases, abnormal protein glycosylation is accompanied by the occurrence. Lifeasible provides services of glycosylation analysis of insect proteins to detect high-throughput glycosylated insect proteins with low content and wide dynamic range based on mass spectrometry and enrichment processing.
| Analysis content | Detail |
| Glycosylation analysis of insect proteins |
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Lifeasible uses mass spectrometry and enrichment processing to perform high-throughput detection of glycosylated insect proteins with low content and wide dynamic range. Special marking is carried out during the sugar cutting experiment to avoid false positive results. After the protein is enzymatically hydrolyzed, the N-glycosylated peptides are enriched by lectin, and then the sugar chain is cut off by N-glycoamidase (PNGase) in heavy water, and then the glycosylation site is analyzed by LC-MS/MS. Point analysis, to achieve large-scale N-glycosylated protein qualitative and quantitative analysis.
Fig 1. Service flow for glycosylation analysis of insect proteins.
| Sample | Requirements |
| Fresh tissue dry weight | >40 mg, transport using dry ice |
| Protein solution | >400 µg, transport using dry ice |
| Number of freshly cultured cells | >2×107, transport using dry ice |
Lifeasible provides services of glycosylation analysis for insect proteins to detect high-throughput glycosylated insect proteins with low content and wide dynamic range based on mass spectrometry and enrichment processing. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.
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