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Insect Active Ingredient Detection

Insect Active Ingredient Detection

Insects contain active substances such as flavonoids, alkaloids, polysaccharides, hormones, and phospholipids that have health-care functions for the human body. Natural bioactive substances have physiological activities such as anti-inflammatory, anti-cancer, and antioxidant. Insects or some insect secretions are rich in biologically active substances, including proteins, fats, hormones, chitin, vitamins, mineral elements, etc., which have a wide range of potential applications.

Fig 1. Main bioactive substances in insects-Lifeasible.Fig 1. Main bioactive substances in insects.

Insect bioactive substances are involved in all physiological activities of insects, with the wide range of applications of active substances in insects, Lifeasible provides general biological activity testing services for insects to ensure they are qualified. We can provide testing services for total flavonoids, various esters, total dietary fiber, and mineral elements.

Our Services for Insect Active Ingredient Detection

Service Content Methods
Total flavonoids Liquid chromatography-mass spectrometry (LC-MS)
Lifeasible offers the technology of LC-MS to detect total flavonoid in insect samples. Coupled LC-MS systems are popular in total flavonoids analysis. The absolute quantification of flavonoids was performed by LC-MS.
Total dietary fiber Biochemical extraction
Lifeasible use biochemical extraction to detect total dietary fiber in insect sample like live insect, insect carcasses. This method can detect total dietary fiber accurately through heat stabilization and extraction by ethanol, the residue was washed with ethanol and acetone, dried and weighed, then we get the total dietary fiber residue.
Mineral elements
  1. Zinc
  2. Calcium
  3. Selenium/ Total selenium
  4. Iron
Inductively coupled plasma atomic emission spectroscopy (ICP-AES)
Lifeasible offers ICP-AES to detect mineral elements such as zinc, calcium, selenium/total selenium and Iron. Based on inductively coupled plasma atomic emission spectroscopy technology, it provides conventional mineral ions and heavy metal ions, and the standard curve is drawn by standards to achieve absolute qualitative quantification of target substances in insect samples.
Various esters Gas chromatography-mass spectrometry (GC-MS)
We combine the features of GC-MS to identify different substances within an insect sample. The method is compatible and accurate for the determination of medium and long chain fatty acid content in samples.
  1. C22:6n3
  2. C18:3n6
  3. C18:2n6c
  4. C18:1n9c
  5. C16:0
  6. C12:0
  7. C13:0
  8. C14:0
  9. C14:1
  10. C16:1
  11. C18:0
  12. C18:1n9t
  13. C18:2n6t
  14. C20:1
  15. C20:2
  16. C20:3n6
  17. C20:5n3
  18. C21:0
  1. C22:0
  2. C22:1n9
  3. C22:2n6
  4. C23:0
  5. C24:0
  6. C24:1
  7. C18:3n3
  8. C20:4n6
  9. C4:0
  10. C6:0
  11. C8:0
  12. C10:0
  13. C11:0
  14. C15:0
  15. C15:1
  16. C17:0
  17. C17:1
  18. C20:0
  19. C20:3n3

Sample Requirements

Insect active ingredient detection can be used for the live insects and insect carcasses. The number of samples is not less than 9 and each sample weights not less than 0.6 mg.

Advantages

  • Getting the absolute quantification of flavonoid in insect samples.
  • Absolute qualitative quantification of target mineral elements in insect samples.
  • Compatible and accurate for the determination of medium and long-chain fatty acid content like various esters in samples.

Lifeasible provides active ingredient detection in insects with absolute qualitative of samples' determination and compatible and accurate of content by experienced scientists, researchers, and technicians. If you are interested in our active ingredient detection services or if you have any questions, please click online inquiry for more detailed information about the detection of total flavonoids, various esters, total dietary fiber and mineral elements in insect samples.

Reference
  1. Chen, X.W.; et al. Analysis of nutrient components of four insects [J]. Feed Research. 2012(04): 80-82.
Our Services are for Research Use Only. Not for Clinical Use!
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