Insect Hormone Detection

Holometabolous insects undergo multiple developmental stages from embryo to adult throughout their lifecycle. Insects can only complete individual growth and development through periodic molting. The hormones primarily regulating insect molting and metamorphosis are ecdysone and juvenile hormone, which act synergistically to regulate the cascading reactions and interactions within gene networks.

• Juvenile hormone (JH) is an important hormone secreted by the pharyngeal glands during insect development. Its functions in insect growth and development include inhibiting the emergence of adult characteristics during the larval stage and promoting adult reproductive development.
• Ecdysteroids are naturally occurring steroid that initiates and regulates molting and metamorphosis. Its functions include promoting cell growth, enhancing cellular metabolism, and acting as an effective substance for cell activation, with excellent exfoliating properties.

Lifeasible specializes in insect research. With our advanced technical platforms and experienced expert team, we aim to provide comprehensive testing solutions for insect hormone detection.

What are the Detection Indicators?

• 20-Hydroxyecdysone 20E
• Juvenile hormone I JH I
• Juvenile hormone II JH II
• Juvenile hormone III JH III

Whether it is common insect hormones or rare special hormones, we can provide professional testing services based on customer needs to meet the diverse research and application needs of customers in different fields.

Our Advanced Technology Platform

We've got top-notch testing equipment, like high-performance liquid chromatography-mass spectrometry (HPLC-MS), gas chromatography-mass spectrometry (GC-MS), and enzyme-linked immunosorbent assay (ELISA) machines. These devices feature extremely high sensitivity and resolution, enabling precise detection of trace amounts of insect hormones in samples, ensuring accurate and reliable testing results. Taking HPLC-MS as an example, it can accurately separate and quantitatively analyze insect hormones in complex biological sample matrices, with detection limits as low as nanograms or even picograms, providing a robust foundation for high-precision testing services.

Application Areas of Insect Hormone Detection

• Research on the physiological mechanisms of insect growth and development, diapause, metamorphosis, and reproduction.
• Research on the mechanisms of biotic and abiotic stress.
• Research on the mechanisms of plant diseases, insect pests, and homeostasis.
• Development and application of biological pesticides, etc.

Sample Requirements

• Whole insects. Rinse the whole insects thoroughly, use absorbent paper to remove surface liquids, ensure each sample weighs approximately 200 mg or more, freeze rapidly in liquid nitrogen, then store at -80°C, and ship with dry ice.
• Tissue. Cut the target tissue sample, take approximately 200 mg or more of tissue per sample, wash the tissue with physiological saline, use absorbent paper to dry the surface liquid, freeze rapidly with liquid nitrogen, transfer to a -80°C freezer for storage, and ship with dry ice.
• Hematolymph. Centrifuge to collect approximately 50 μl or more of hematolymph per sample, freeze rapidly with liquid nitrogen, transfer to a -80°C freezer for storage, and ship with dry ice.

Our Testing Process

• Online consultation. Customers can place orders online through our official website or call our customer service hotline for consultation.
• Sample collection and transportation. We will provide customers with detailed sample collection guidelines. After collecting samples according to the guidelines, customers should send the samples to us.
• Sample testing and data analysis. Upon receiving the samples, our testing personnel will immediately register and pre-process them, then conduct instrument analysis and experimental operations according to the established testing protocol. During the testing process, data collection and recording will be conducted simultaneously, and the data will be processed and analyzed.
• Report generation and delivery. After testing is completed, we will prepare a detailed testing report based on the analysis results. Additionally, we provide further technical support and consultation services.

Fig.2 Our experimental process. (Lifeasible)

Why Choose Us?

• Precise detection. We can perform highly sensitive and accurate detection of insect hormones to ensure reliable and accurate detection results.
• Comprehensive detection projects. We provide detection services for a variety of insect hormones, covering various important hormones in the growth and development process of insects, to help customers gain a deeper understanding of insect physiological status and ecological behavior.
• Fast and efficient detection process. We continuously optimize the detection process to enable customers to obtain detection results promptly and quickly advance their scientific research projects.
• Customized solutions. We provide customized testing solutions tailored to the specific needs of each client project, ensuring that unique requirements are met.

Lifeasible is committed to becoming a leading provider of insect hormone testing services in the industry, offering professional, efficient, and precise testing solutions to clients such as research institutions, agricultural enterprises, and environmental protection organizations, thereby supporting their progress in insect research and application fields. If you are interested, please feel free to contact us.