The identification of insect proteins is the basis for insect proteomics research, using mass spectrometry to identify all or specific insect proteins in cells, tissues, body fluids or protein gels/liquids after isolation, purification and enrichment, to provide a full understanding of insect protein species in a particular environment, and to provide visual protein identification for the study of disease development and insect growth and development. The product is designed to provide a visual identification of protein species for the study of disease development and insect growth and development.
Insect protein identification products can be divided into two categories, depending on the subject and content of the study, protein full-spectrum analysis and gel spot/ strip identification. Lifeasible provides insect protein identification analysis services to study the expression of insect proteins in specific environments, the development of diseases in insects and the expression of specific proteins in the growth and developmental state of insects, and provide powerful support for insect research.
Analysis content | Detail |
Analysis of standard information |
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Personalized information analysis |
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Customized information analysis |
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Lifeasible uses full-spectrum of protein to analyze the insect proteome as a whole, using mass spectrometry to obtain as much protein information as possible. We use this method to provide a comprehensive analysis of the protein species contained in unknown insect samples. Mass spectrometry-based protein full-spectrum analysis provides reference information for high-throughput quantitative and modification analysis of proteins. In addition, we combine the full-spectrum analysis data with transcriptome data to complement each other and jointly validate gene annotation and functional information.
We use bi-directional electrophoresis to obtain protein profiles, analyze them with relevant software to obtain differences or protein spots of interest, and use mass spectrometry to identify them and obtain detailed information about the protein.
The specified bands or lesser fractions of protein solutions isolated from SDS-PAGE gels are identified by mass spectrometry.
Fig 1. Service flow for insect protein identification analysis.
Sample | Requirements |
Fresh animal tissue dry weight | >10 mg |
Protein solution | >200 µg (0.5 µg/ µL) |
Number of freshly cultured cells | >107 |
Komas blue staining, silver-stained gel spots/ strips | Visible spots to the naked eye; >1µg protein |
Lifeasible provides insect protein identification analysis services to study the expression of insect proteins in specific environments, and specific insect proteins in the growth and developmental state of insects, in order to provide powerful support for insect research. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.