Molecular Marker Technology of Bombyx Mori

Bombyx mori is an insect from the moth family Bombycidae. The silkworm is the larva or caterpillar of a silk moth. It is an economically important insect, being a primary producer of silk. A silkworm's preferred food are white mulberry leaves, though they may eat other mulberry species and even the osage orange. Bombyx mori are entirely dependent on humans for reproduction, as a result of millennia of selective breeding.

Our Services for Molecular Marker Technology of Bombyx Mori

Bombyx mori is an important economic insect. With the development of molecular breeding technology, many genes related to important traits have been identified in bombyx mori by various molecular markers, including five aspects of growth and development, immunity, sex determination, disease resistance, and genome-wide selection for breeding. Lifeasible provides molecular marker services for bombyx mori with Inter-Simple Sequence Repeat (ISSR), Amplified Fragment Length Polymorphism (AFLP), and Random Amplified Polymorphic DNA (RAPD) help to select and breed the new varieties of these silkworms.

• Analysis of the relatedness of bombyx mori variety resources based on ISSR molecular markers

The ISSR molecular marker technology was applied to analyze the relatedness of bombyx mori species resources, which can provide a basis for the rational conservation and utilization of bombyx mori germplasm resources and the selection of breeding parents.

• Application of RAPD technology in genetic breeding of bombyx mori

The genomic DNA of bombyx mori was amplified to analyze its genetic purity and to detect the frequency of polymorphic bands among individuals. Stable amplification of DNA-specific fragments using the screened RAPD random primer fragments for accurate and rapid identification of breeds and purity. Analysis of genetic polymorphisms and their affinities in bombyx mori breeds. To study the DNA polymorphism of bombyx mori breeds.

• AFLP fingerprinting of the original bombyx mori species based on AFLP

Analysis of the varietal affinities of bombyx mori by means of AFLP.

Our Methods for Molecular Marker Technology of Bombyx Mori

• ISSR

Lifeasible offers ISSR molecular marker technology to analyze the relatedness of bombyx mori species resources, which can provide a basis for the rational conservation and utilization of bombyx mori germplasm resources and the selection of breeding parents. We amplify bands from the nymphal genomic DNA of bombyx mori varieties with the screened ISSR primers to observe the genetic diversity among bombyx mori varieties.

• AFLP

Lifeasible provides AFLP molecular marker technology to construct AFLP fingerprint profiles of high quality and robust bombyx mori progenitors and to analyze their genetic similarity coefficients and relatedness. We use AFLP primers to amplify the bands and construct AFLP fingerprints for the identification and detection of bombyx mori at the molecular level.

• RAPD

Lifeasible uses RAPD for DNA fingerprinting and species identification of bombyx mori, analysis of relatedness among different geographical species of bombyx mori, gene localization, and construction of molecular linkage map of bombyx mori.

Applications

• Species identification and detection of bombyx mori.
• Reveal the genetic differences and affinities among bombyx mori varieties.
• Construct the molecular phylogenetic tree of bombyx mori to provide basic information for the selection and breeding of new varieties of bombyx mori. Rapid development of a productive bombyx mori breeding program with the guidance provided by molecular linkage mapping.

Lifeasible provides molecular marker services for bombyx mori by ISSR, AFLP and RAPD to identify and detect the species, construct the molecular linkage map, and provide basic information for the selection and breeding of new varieties of bombyx mori. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.