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Philosamia cynthia ricini is a subspecies of the Simarouba silkworm in the family Macrobrachium. It is one of the most important silk-producing silkworm species. After refining the cocoon, it becomes an excellent industrial raw material for silk spinning. The nature of silk is basically similar to the domestic silkworm, and good elasticity, after carding can be obtained from 1 to 4 levels of long fiber 60.9%, short fiber 35.3%, the loss of only 3.8%. Philosamia cynthia ricini has the advantages of fast development, easy rearing, strong disease resistance, robust silkworm, backlight swarming, etc. It has become an economic insect and can also be used as good material for genetic research.
Philosamia cynthia ricini is a kind of silk-spitting economic insect with multiplicity and no stagnation period under suitable environmental conditions. The application of marker selection for molecular screening of varieties for castor silkworm, especially in the production of superior varieties, appears to be particularly urgent. Lifeasible provides molecular marker services for philosamia cynthia ricini with Inter-Simple Sequence Repeat (ISSR), and Simple Sequence Repeats (SSR) to aiding breeding of philosamia cynthia ricini.
| Service content | Detail |
| Analysis of genetic diversity of philosamia cynthia ricini species based on ISSR |
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| Identification of germplasm resources of philosamia cynthia ricini and analysis of genetic variation of philosamia cynthia ricini based on SSR molecular markers |
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Lifeasible use ISSR molecular markers to reveal the genetic diversity of philosamia cynthia ricini. We amplify a segment of genomic DNA sequence with reverse aligned SSR on both sides by adding anchor purine (not more than 4) or pyrimidine bases at the 5' end or 3' end of the SSR and then use this as primer, which has the advantages of easy operation, high reproducibility, less DNA template usage and rich polymorphism.
SSR molecular markers of philosamia cynthia ricini and their applications belong to the field of molecular biology and silkworm breed identification technology. Lifeasible obtains genomic SSR molecular marker locus information by analyzing the genome sequence of philosamia cynthia ricini and designs and synthesizes amplification-efficient and polymorphism-rich SSR primers with high amplification efficiency and rich polymorphism were designed. The SSR fingerprint profile of the castor silkworm was constructed using philosamia cynthia ricini germplasm as the material. The detection of SSR markers can be accomplished by PCR amplification and electrophoretic detection techniques, which can be used for the identification of philosamia cynthia ricini germplasm resources and reveal the genetic variation of each strain at the DNA level, and also for the development and application of specific markers.
Lifeasible provides molecular marker services for philosamia cynthia ricini by ISSR and SSR to analyze the genetic diversity, germplasm diversity and identify species of philosamia cynthia ricini to aiding breeding. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.
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