Protein Expression in Insect Cell Systems

The insect baculovirus vector expression system is widely used for the preparation of various recombinant proteins, with the advantages of high expression level of exogenous genes, easy expression of heterologous multimeric proteins, safety, etc. The expressed recombinant proteins usually have correct post-translational modification and biological activity, insect cells grow in suspension, easy to scale up culture, which is conducive to large-scale expression of recombinant proteins. With its stable experimental system, high expression level and post-translational modification function, it is generally recognized as a powerful tool for expressing large-scale proteins.

Our Services for Protein Expression in Insect Cell Systems

The Baculovirus expression vector system (BEVS) is widely recognized as a powerful tool for expressing large-scale proteins, providing highly active recombinant proteins and solving protein activity difficulties that cannot be solved in other systems, due to its stable experimental system, high expression level and post-translational modification. Lifeasible provides protein expression services in insect cell systems including Bac-to-Bac Baculovirus Expression System and Baculovirus Expression System. In addition, the Lifeasible insect protein expression service is complemented by free gene optimization, protein design and analysis services, and expression protocol advice, which will save you a lot of time and money.

The Lifeasible baculovirus-insect cell system uses the classical Bac-to-Bac baculovirus expression system, which can optimize gene sequences and design experimental protocols to help customers complete a variety of cytokines, kinase proteins, multi-structural domain proteins, and processing modified proteins according to their needs. We also offer Baculovirus expression system with higher expressed protein expression, highly consistent results, and more streamlined steps to shorten experiment time.

Advantages

• Express large molecular weight protein, such as 250 KD protein expression, still better stability, no breakage or degradation.
• Insect cells are relatively simple compared to mammalian cell culture, 27 degrees culture, no CO₂ supplementation, insect expression system cost is lower than the mammalian expression system.
• The virus is encapsulated once, used for a long time, and expressed stably. Compared with the mammalian transient expression system, this system does not need to extract a large number of plasmids, but only needs to encapsulate the virus and do a good job of post-expression detection to stabilize the amplified expression.
• Insect cell system can carry out post-translational processing modifications, including glycosylation, phosphorylation, acylation, signal peptide excision and peptide cleavage and disassembly, etc.
• Multiple genes can be expressed simultaneously, such as antibodies, fab, proteins that interact with each other, etc.
• Baculovirus is not infectious to vertebrates.
• Express toxic proteins, such as antibacterial peptides.

Lifeasible provides protein expression services in insect cell systems including Bac-to-Bac Baculovirus Expression System and Baculovirus Expression System. We also offer free gene optimization, protein design and analysis services, and expression protocol advice by our professional team. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.