Qualitative Analysis of Modified Proteins in Insects

Qualitative protein analysis is the process of determining whether, and what kind of protein is present in a sample. Protein characterization generally refers to protein identification and sequence analysis using mass spectrometry. Protein qualitative analysis is divided into two strategies: top-down analysis and bottom-up analysis. At present, the bottom-up analysis strategy is more widely used in protein qualitative analysis. With the rapid development of mass spectrometry, the qualitative analysis of proteins using mass spectrometry has been more widely used and studied.

Our Services for Qualitative Analysis of Modified Proteins in Insects

Bottom-Up refers to the identification of proteins by analyzing peptides generated by enzymatic digestion of proteins. The top-down strategy directly identifies intact proteins and has some potential advantages in terms of post-translational modifications and protein isotrope identification. By far the most common post-translational modifications of proteins are phosphorylation, ubiquitination, nitrosylation, methylation, acetylation, sulfation, glycosylation, and acylation, and there may be many more modification types yet to be discovered.

Lifeasible provides services for qualitative analysis of modified proteins in insects based on the property of LC-MS/ MS. We can offer Analysis of Protein Modification Sites in Insects-detect the specific sites of modifications occur on insect proteins or peptides and Analysis of Protein Modification Expression Profiles in Insects-analyze the expression profiles of different proteins in different growth, development and metabolic processes of insects.

Our Methods for Qualitative Analysis of Modified Proteins in Insects

Lifeasible uses ultra-high resolution mass spectrometry technology to provide qualitative and quantitative analysis services for insect proteins. Through insect protein extraction, SDS-PAGE, insect proteolysis, enrichment of modified peptides, LC-MS/ MS analysis, data retrieval and biological Information analysis and other steps, we can analyze the different modification sites of insect proteins and the expression profiles of different modified proteins under different conditions or different growth and development stages, and strive to solve the impact of insect modified proteins on insect physiological functions.

Advantages

For the analysis of modification sites, our advantage is to scan three conventional modification types at one time and perform personalized targeted modification analysis based on the determined amino acid sites and modification types. For the analysis of modified protein expression profiles, our advantage is the high specificity of peptide enrichment.

Lifeasible provides services for qualitative analysis of modified proteins in insects by our professional team with LC-MS/ MS. We offer two types to detect the specific sites of modifications occur on insect proteins or peptides and detect various expression profiles of different proteins in the growth development of insects. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.