Target Bisulfite Sequencing of Bombyx Mori

Bombyx mori is an important model insect, and it is also an important domesticated economic animal. DNA methylation plays a very important role in regulating gene expression of bombyx mori. The significance of DNA methylation in bombyx mori is multifaceted, and most DNA methylation is essential for normal development. It plays a very important role in many key processes, including disease development. DNA methylation in different genomic regions may have different effects on gene activity.

Our Services for Target Bisulfite Sequencing of Bombyx Mori

Based on the whole genome methylation sequencing technology, the DNA methylation sequencing technology of target genes has gradually developed into an important and indispensable technology, which is very important for the methylation verification of specific regions. Lifeasible provides Target Bisulfite Sequencing (Target-BS) services for bombyx mori to validate methylation of subsequent target genes.

Target-BS, using methylation-specific multiplex PCR primer design software, multiplex amplification of target genes after sulfite conversion or oxidative sulfite conversion, and ultra-high depth (above 1000X) after library construction accurate detection of methylation and/or hydroxymethylation.

Analysis content	Detail
Data quality control	Low quality filtering Connector filtering Output statistics Allegation Evaluation
Sequence alignment	Sequence alignment using instrument software
CG depth statistics and methylation level calculation	Single-sample CG site statistical depth and methylation level statistics Matrix of methylation levels at CG sites in all samples
Visualization of methylation levels at amplicon CG sites	Graph Heatmap

Our Methods for Target Bisulfite Sequencing of Bombyx Mori

Samples were subjected to targeted bisulfite sequencing followed by independent validation of selected sample differentially methylated regions using target gene DNA methylation sequencing. Using the methylation-specific multiplex PCR primer design software, the target gene after sulfite conversion is multiplex amplified, and the ultra-high-depth (above 1000X) methylation detection is carried out after the library is established.

Service Flow

Fig 1. Service flow for Target-BS of bombyx mori.

Sample Requirements

Sample	Requirements
Fresh tissue	>20 mg
Total DNA	>50 ng, no significant degradation of DNA or protein contamination, as judged by gel electrophoresis on of DNA, gel electrophoresis test gel chart required.
Number of freshly cultured cells	>10⁶
Whole blood	>0.5 mL, whole blood samples should be used in normal EDTA anticoagulation tubes, avoid using heparin anticoagulation tubes; cell samples should be live cell frozen samples

Applications

Target-BS is widely used for methylation biomarker screening and validation of known sites in bombyx mori.

Advantages

Low sample demand, 20 ng genomic DNA can achieve multi-gene amplification.
Multiple gene multiple amplification, high detection throughput.
Ultra-high depth sulfite methylation sequencing, with high detection sensitivity and accuracy, can accurately detect methylation levels below 1% in samples.

Lifeasible provides Target-BS services for bombyx mori by establishing a flexible targeted methylation sequencing technology, methylation detection of bombyx mori target genes/CpG sites. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.

Our Services are for Research Use Only. Not for Clinical Use!

Services

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