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In the post-genomics era, proteins, the expression products of genes, play an important role in the collective physiology and pathology of organisms as the most direct performers and embodiment of biological functions. High-throughput proteomics research technologies can screen for potentially valuable biomarkers and targets in these processes. However, large-scale protein expression profiling has not significantly improved our understanding of biomarkers for a long time, mainly due to the lack of sufficiently rapid and scaled-up validation of differential protein results.
Proteins often do not perform their functions alone and need to combine with other proteins to form complexes to function. Protein-protein interactions form a major component of the cellular biochemical response network, and protein-protein interaction networks and transcriptional regulatory networks are important for the regulation of cells and their signaling. Verification that proteins can interact with each other also allows further studies of protein function. Lifeasible provides verification services for insect interacting proteins to achieve rapid validation of candidate proteins accelerates the study of insect protein function and provides support for revealing the regulatory mechanisms of insect proteins on their own development.
We can provide verification of insect interacting proteins in vitro and in vivo. The advantages and disadvantages of the specific methods are listed in the table below, and you can choose the method according to your needs.
| Service content | Methods | |
| In vivo validation | Yeast two-hybrid system | Advantages: simple and easy to operate. Disadvantages: high false positives. |
| Bimolecular fluorescence complementation | Advantages: fast and intuitive and highly sensitive. Disadvantages: high false positives and inability to observe the interaction process in real time. |
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| Co-Immunoprecipitation | Advantages: the interaction of proteins takes place in a natural state, which avoids artificial influences. Disadvantages: the binding of two proteins may not be direct, but there may be a third party acting as a bridge in the middle; it is necessary to predict what the target protein is before the experiment to select the final antibody to be detected, so if the prediction is not correct, the experiment will not get the result. |
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| In vitro validation | GST-pull down | Advantages: direct protein-protein interactions can be verified; GSH glutathione-coupled spheres have high affinity and high elution purity. Disadvantages: does not fully reflect the true intracellular protein interactions; fusion expression of GST tags may alter the original folding structure of the original target protein. |
| Microscale thermophoresis | Advantages: the amount of sample is small; there is no need to use antibodies, and there is no need to obtain high-purity proteins; not only can the interaction relationship between two biomolecules be qualitatively determined, but also the binding affinity can be quantitatively judged. Disadvantages: no kinetic information and concentration analysis; if the interacting protein forms a complex with other molecules or competes with other molecules, the binding data will be affected; if the protein has post-translational modification, the data detected by different systems will drift; if the protein to be tested is not stable in the experimental system, which will affect the stability of the data. |
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| Biacore | Advantages: no labeling; high sensitivity; rapid detection; real-time quantitative testing. Disadvantages: high cost. |
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Fig 1. Service flow for the verification of insect interacting proteins.
Lifeasible can provide both in vivo and in vitro validation of proteins, with a variety of methods for customers to choose from.
Lifeasible provides verification services for insect interacting proteins with several methods to achieve rapid validation of candidate proteins accelerates the study of insect protein function by our professional team. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.
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