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Validation of Insect Interaction Molecules

Validation of Insect Interaction Molecules

In the field of life sciences, the interaction between biomolecules plays a very important role. The analysis of molecular interactions can not only elucidate cell biological events, but also provide the basis for disease pathogenesis and drug discovery. Protein and nucleic acid are two important substances that make up living organisms. Protein is the product of gene expression, gene expression is inseparable from protein. They depend on each other and restrict each other. Many important biological processes in biological systems, such as DNA synthesis, gene transcription activation, protein translation, modification and localization, and information transduction, all involve the role of protein complexes.

Our Services for Validation of Insect Interaction Molecules

Discovering and verifying the interaction of proteins and nucleic acids, proteins and proteins in living organisms is the first step in understanding their biological functions. Lifeasible provides validation services for insect interaction molecules include Validation of Insect Interacting Proteins-to study the function of insect protein and Validation of Insect Protein-Nucleic Acid Interaction-to analyze the interaction between protein and nucleic acid. We aim to help explain the function of the molecule and related regulatory mechanisms in insect.

We can provide the verification of insect interacting proteins and insect protein-nucleic acid interaction. The method corresponding to each item is in the table, and you can choose the service items according to your needs.

Service content Methods
Validation of insect interacting proteins In vivo validation
  • Yeast two-hybrid system
  • Bimolecular fluorescence complementation
  • Co-Immunoprecipitation
In vitro validation
  • GST-pull down
  • Microscale thermophoresis
Validation of insect protein-nucleic acid interaction Protein-DNA interaction
  • DNA pull-down
  • Yeast one-hybrid
  • Dual luciferase
  • Chromatin immunoprecipitation
Protein-RNA interaction
  • RNA pull-down
  • RNA binding protein immunoprecipitation

Our Methods for Validation of Insect Interaction Molecules

We use several methods to validate the interation between proteins and proteins of insect. We offer the validation of interating proteins in vivo by yeast two-hybrid, bimolecular fluorescence complementation and co-Immunoprecipitation, and in vitro by GST-pull down and microscale thermophoresis. We also use some methods to validate the interaction between protein and nucleic acid include protein-DNA and protein-RNA. We use DNA pull-down, yeast one-hybrid, dual luciferase and chromatin immunoprecipitation to validate the interaction of protein-DNA, and RNA pull-down and RNA binding protein immunoprecipitation to validate the interaction of protein-RNA.

Service Flow

Fig 1. Service flow for the verification of insect molecules interactions- Lifeasible.Fig 1. Service flow for the verification of insect molecules interactions.

Advantages

Lifeasible offers the validation of molecule interactions of insect with several methods. We can validate the interaction between protein and protein both in vivo and in vitro, and offer the validation of protein-DNA interactions and protein-RNA interactions. You can choose the validation approach according to your needs.

Lifeasible provides validation services for insect interaction molecules include Validation of Insect Interacting Proteins and Validation of Insect Protein-Nucleic Acid Interaction to help explain the function of the molecule and related regulatory mechanisms in insect by our professional team. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.

Our Services are for Research Use Only. Not for Clinical Use!
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