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Nucleic acid-protein interaction plays an extensive and important role in life activities, and the cooperation between the two is the basis of various life phenomena. Linking the interactions of biological macromolecules such as proteins and nucleic acids in cells or organelles is the core content of comprehensive research on a complete biological pathway. For nearly half a century, researchers have conducted a lot of exploration in the formation and decomposition of nucleic acid-protein complexes, and developed a series of methods and technologies to study their interaction.
The interaction between protein and nucleic acid exists in all levels of gene expression in organisms, and there is interaction between protein and nucleic acid at the level of replication, transcription and translation. Lifeasible provides validation services for insect protein-nucleic acid interactions to analyze the interaction between protein and nucleic acid, so as to help explain the function of the macromolecule and related regulatory mechanisms.
We provide verification services for the interaction between insect proteins and nucleic acids, accurately revealing the mechanism of action between them, and expect to provide a basis for the study of multiple key cellular processes, including transcription, translation, gene expression regulation, recognition, replication, recombination, repair, nucleic acid packaging, chromatin remodeling, and organelle formation and other important life processes. to achieve rapid validation of candidate proteins accelerates the study of insect protein function and provides support for revealing the regulatory mechanisms of insect proteins on their own development.
We can provide the verification of the interaction of protein-DNA and protein-RNA. The advantages and disadvantages of the specific methods are listed in the table below, and you can choose the method according to your needs.
| Service content | Methods | |
| Protein-DNA interaction | DNA pull-down | Advantages: enrichment analysis of low-abundance proteins; easy probe preparation. Disadvantages: Presence of non-specific binding phenomenon; strict reaction conditions |
| Yeast one-hybrid | Advantages: detect weak protein-DNA interactions. Disadvantages: undetectable protein-DNA interactions that depend on post-translational modifications. |
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| Dual luciferase | Advantages: superior sensitivity; low endogenicity; luciferase assay is not affected by other intracellular substances; easy detection. Disadvantages: low consistency of results. |
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| Chromatin immunoprecipitation | Advantages: study DNA-protein interactions in vivo. Disadvantages: highly specific antibodies against the target protein or specific modified tags are required; false negative and false positive signals occur. |
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| Protein-RNA interaction | RNA pull-down | Advantages: enrich and detect low-abundance RBPs-RNAs complexes; directly transcribe target RNA in vitro as bait, avoiding non-specific results caused by RNA complementary probes. Disadvantages: RNA pull-down is an in vitro experiment with a high risk of non-specific binding. |
| RNA binding protein immunoprecipitation (RIP) | Advantages: RIP technology can reflect the interaction between RNA and protein in the natural cell state; identify RNA that directly/indirectly interacts with RBP; RIP-q-PCR verifies the protein screened by RNA pull down-MS to avoid false positives. Disadvantages: proteins that can interact need to be predicted in advance. |
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Fig 1. Service flow for the verification of protein-nucleic acid interactions.
Lifeasible can provide validation of both protein-DNA interactions and protein-RNA interactions. You can choose the method validation approach according to your needs.
Lifeasible provides validation services for insect protein-nucleic acid interactions to analyze the interaction between protein and nucleic acid by our professional team. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.
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