Isolation and Purification Services for Exosomes

Lifeasible offers custom exosome isolation and purification services for the isolation and purification of high-quality exosomes from multiple sources. Isolation methods have been developed to improve the quantity and quality of exosomes and the reproducibility of results obtained using exosome agents. Determining the purity of exosome samples is an essential step before exosome technology can be used in a clinical setting, as the presence of impurities in exosome samples may have undesirable side effects. Currently, we extract exosomes from cell culture medium supernatants or plasma serum mainly by ultracentrifugation, which is the most widely accepted exosome extraction method in exosome research.

About the Separation Techniques of Ultracentrifugation

• Differential Ultracentrifugation

Differential ultracentrifugation refers to the sequential separation of different extracellular components of a fluid sample according to density, size, and shape at a certain centrifugal force. The protocol involves multiple steps of centrifugation and ultracentrifugation. The first step is designed to eliminate dead cells and bulky apoptotic debris and is adjusted at low spinning speeds. The following steps usually involve higher rotational speeds, depending on the experiment. Differential centrifugation is suitable for large volume preparations, requires minimal technical means, is inexpensive, and has a low risk of contamination from additional separation reagents.

Figure 1. Schematic representation of differential ultracentrifugation-based exosome isolation. (Yang et al., 2020)

• Isopycnic Density Gradient Ultracentrifugation

This method separates particles based on their size and density and can effectively purify extracellular vesicles, and even aggregates, from proteins. The yield of exosomes obtained by density gradient ultracentrifugation has a higher purity.

Figure 2. Schematic representative of gradient density ultracentrifugation-based exosome isolation. (Yang et al., 2020)

Our Services

Lifeasible's experimental team has extensive experience in exosomes isolation and purification, and we have developed a wide range of protocols for the isolation and purification of exosomes from a variety of samples, providing customized solutions for each project with high purity and optimal recovery results, and compatibility with any downstream processing or analysis. Our team can isolate exosomes using various techniques that can be applied to large volume samples.

Advantages of Our Services

• High purity - high removal rate of impurity protein, no introduction of exogenous impurity contamination, to ensure the accuracy of subsequent studies from the source.
• Good particle homogeneity - recovery of a fixed size range of extracellular vesicles, product size range is narrow.
• Structural integrity - maintain intact morphology and function.
• Good reproducibility - highly uniform operation procedure, the experiment is less affected by fluctuation of external factors.
• Good compatibility - compatible with various biological fluids such as blood, cell supernatant, urine, cerebrospinal fluid, pleural fluid, ascites, etc.
• Short time-consuming - simple method, less process, short time required.
• Easy to operate - no special equipment required.

If you are interested in our services, please feel free to contact us.