Untargeted metabolomics is the unbiased detection of the dynamic changes of most of the small molecule metabolites in the body before and after the external stimulation of the plant body by liquid chromatograph mass spectrometer (LC-MS), and the screening of differential metabolites by bioinformatics analysis, and the differential metabolite pathway analysis to reveal the physiological mechanisms of the changes. Lifeasible provides high-quality LC-MS metabolomics assay services based on advanced technology platforms and an experienced team of experts to help plant science research progress.

Our Service for LC-MS Metabolome Assay

We utilize mass spectrometry technology, combined with database search to identify metabolites in the samples, and bioinformatics methods to study differential metabolites in control and experimental groups, biomarker mining, metabolite functional pathways, and other qualitative and quantitative studies.

Assay platform

We have comprehensive metabolomics liquid chromatography-mass spectrometry (LC-MS) equipment capable of meeting LC-MS non-targeted metabolomic studies. Our advanced technology platform can identify various metabolites in complex samples, in which trace metabolites can also be detected, thus providing a more comprehensive metabolite profile.

Sample processing

To date, we have accumulated extensive experience in sample handling through projects covering a wide range of species and sample types. The plants we have tested are not limited to rice, cucumbers, potatoes, soybeans, apples, grapes, tomatoes, and more.

Cloud analysis

We have a cloud platform for histology analysis, including powerful image editing functions and a user-friendly interactive analysis interface, which helps our clients to study their projects quickly.

Our Process for LC-MS Metabolome Assay

Our experimental process includes sample collection and pre-processing, metabolite extraction, on-line testing, library search, and data analysis. We strictly control each step of the experiment and realize standardized operation to ensure high-quality product data.

Fig.1 Our service process. (Lifeasible)

Sample Request

• Sample type. Plant tissues, root secretions, etc.
• Biological replicates. 6 biological replicates are recommended.
• Sample requirement. Plant tissue ≥200mg, liquid soup type ≥ 5ml.
• Sample preparation principles. Principle of representativeness, the principle of promptness, the principle of separate parts, the principle of contamination control, and the principle of low temperature.
• Preservation and transportation. Samples can be flash-frozen with liquid nitrogen and stored at -80 °C or sent directly on dry ice.

Application of LC-MS Metabolome Assay

• Resistance-related studies. The study of resistance mechanisms in plants subjected to stress conditions such as high temperature, high salt, and drought at some growth stage.
• Breeding mechanisms. Mechanism of action of metabolites and lipid compounds during seed development of crops.
• Plant growth and development. Mechanisms of metabolic regulation in plants during growth.

Highlights of Our LC-MS Metabolome Assay

• We have professional non-targeted metabolomics experts and a strong information analysis team, which can provide personalized analysis services according to customer needs.
• We have a high-throughput database of multiple standards, ensuring our test results' accuracy and reliability. This database can also support the analysis of diverse samples, enabling customers to obtain more comprehensive metabolite information.
• We utilize a high-resolution mass spectrometry platform, making our analysis industry-leading in sensitivity and resolution.
• Strict quality control measures are implemented during testing to ensure that each batch meets high standards and reduces the potential for experimental error.

Fig.2 Our service advantages. (Lifeasible)

Lifeasible is committed to providing high-quality LC-MS non-targeted metabolomic assays to help researchers advance their research programs. If you are interested, please feel free to contact us.