Leymus chinensis Transformation

Leymus chinensis is a kind of excellent perennial grass, with the characteristics of cold-resistant, drought-resistant, alkali-resistant, barrenness-resistant, and sand-resistant. In recent years, due to the lack of scientific management of Leymus chinensis grassland, as well as overgrazing and other unfavorable effects, coupled with the existence of itself, such as low fruiting rate, low germination rate, and other problems. Leymus chinensis poses a serious threat to the maintenance of Leymus chinensis biodiversity and severely limits the pace of using Leymus chinensis for the construction of artificial grassland and, the improvement of natural grassland, and the management of desertification. Therefore, accelerating the breeding process of Leymus chinensis, using biotechnology, and discovering new germplasm have become the critical directions of Leymus chinensis research.

Lifeasible has established a robust and efficient plant genetic transformation platform, and our experienced team of experts can provide specific genetic transformation methods for different plants according to customers' transformation needs. So far, in the study of Leymus chinensis genetic transformation system, we have successfully established a genetic transformation system for Leymus chinensis by inducing its seeds to produce healing tissues and using these healing tissues as Agrobacterium rhizogenes infestation targets. The Leymus chinensis genetic transformation service we provide can accelerate its trait improvement and also provides a powerful tool for the mining of excellent genetic resources and functional research of Leymus chinensis.

What do we offer?

We used Leymus chinensis mature embryo as the explant to improve the efficiency of Leymus chinensis mature embryo healing tissue induction through the use of new and improved medium formulations, incubation time, and culture conditions, and screened the hormone concentration ratio suitable for the differentiation culture, to obtain a highly efficient and stable system of plant regeneration induced by Leymus chinensis mature embryo. The results of this study will lay the foundation for the establishment of the genetic transformation system of Leymus chinensis and provide technical support for the molecular design breeding and genetic improvement of Leymus chinensis. Based on the establishment of the efficient regeneration system for Leymus chinensis, we have established an Agrobacterium-mediated genetic transformation system for Leymus chinensis by utilizing the transformation method of Agrobacterium rhizogenes and by studying the most suitable conditions for the genetic transformation of Leymus chinensis.

Our services

• Customers provide vectors or entrust us to perform vector construction.
• Obtain Leymus chinensis histocultured sterile healing tissues, use seeds of Leymus chinensis as exosomes, and carry out the induction and succession of healing tissues on the induction medium.
• Agrobacterium-mediated Leymus chinensis genetic transformation, screening.
• Differentiation of positive healing tissues into seedlings.
• Rooting and strengthening of regenerated green seedlings.
• PCR detection of exogenous genes in T0 generation transgenic seedlings.

Our service workflow

Note to Customers

• We have a robust Leymus chinensis genetic transformation platform and can conduct yearly experiments.
• Typical experimental time is 4-6 months, due to exceptional weather conditions, the experimental period may be extended.
• The cost of the experiment may fluctuate according to the situation; please consult us directly for the specific cost.

Experimental results we provide

• Some pictures of genetic transformation and screening.
• Pictures of some samples of differentiation and regeneration of healing tissue.
• Agarose gel electrophoresis of PCR detection of exogenous gene in T0 generation transgenic seedlings.
• 15-20 plants of T0 generation transgenic seedlings.

The realization of Leymus chinensis genetic transformation research greatly facilitates the study of the molecular mechanism of salinity tolerance in Leymus chinensis and the excavation of related functional genes to breed more excellent new varieties of Leymus chinensis at a faster rate and to obtain engineered plants that are tolerant to a wide range of salinities. Lifeasible is an industry leader in plant genetic transformation research, and our specialized technology platform and experienced team of experts ensure the success rate of plant transformation. If you are interested in us, please feel free to contact us.