Liposomes have the following characteristics: (1) targeting; there are four types of chemical targeting, physical targeting, antibody targeting, and receptor targeting; (2) non-toxic safety; lecithin has a high safety level even when used in large amounts; (3) lipid-soluble and water-soluble compatibility: the phospholipid bilayer in the liposome wall material can carry lipid-soluble molecules while the vesicles can carry water-soluble molecules inside. Therefore, when wrapping small molecules, the efficacy of small molecules can be fully utilized.
MicroRNAs are cellular RNA species that block the production of specific proteins by binding to and targeting mRNA for destruction. Due to their small size (i.e., 21-22 base pairs), microRNA mimics in mature double-stranded form can be transferred into the target cell. Liposomes can encapsulate polar or non-polar molecules. Their application can be extended by designing very small liposomes (<100 nm). The physicochemical and biophysical properties of liposomes can be easily manipulated for use in various fields. They can be fine-tuned to provide a rapid or delayed release of the encapsulated molecules.
Lifeasible aims to provide our customers professional liposome development, modification and characterization testing services. Encapsulation of microRNAs in liposomes expands the potential applications of liposomes. We currently have this service component in place, and you can provide us with the material to be encapsulated, which can be small molecules, nucleic acids, etc. We offer a range of liposome services designed to identify synthesis, characterization, formulation (encapsulation), preservation, and cargo delivery as key components of the liposome target product profile. A range of synthesis protocols is used to measure physicochemical properties relevant to product development.
The liposomal material is suspended in ethanol at various concentrations. This solution is then transferred to ethanol, and this stock solution is diluted to prepare a working solution. Once the desired dilution is reached, microRNA is added and incubated at room temperature. The solution is then placed in a separate vial containing PBS, magnetically stirred, and the working solution containing the microRNA is drawn into a glass syringe. Finally, the liposome suspension containing microRNA was dialyzed overnight.
Lifeasible offers a customization service for liposome particle size and can develop a product synthesis protocol according to your requirements.
Lifeasible offers a full range of services, including dynamic light scattering, nanoparticle tracking analysis, microscopy (scanning electron microscopy, transmission electron microscopy) analysis, and microRNA encapsulation rate testing, providing customers with a full range of liposome analyses.
If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.
