Liposomes encapsulate plasmid DNA for efficient DNA delivery, with the advantage of lower production costs and fewer safety concerns. Lifeasible can provide customized solutions for liposome encapsulation services. Based on your requirements, we can provide you with specific liposome services, including anionic liposomes, cationic liposomes, and other functional liposomes. In addition, our experts have advanced liposome preparation techniques and can provide you with liposomes of different sizes. We can also provide a physicochemical characterization of liposomal products, providing you with a full range of services.
The encapsulation of DNA plasmids in liposomes has been achieved by various techniques with varying degrees of success, protecting DNA from nuclease attack and increasing its biological stability and bioavailability. Reverse-phase evaporation produces large monolayers of liposomes (average diameter of 400-500 nm), of which up to 40% can be captured using negatively charged phosphatidylserine (PS) containing vesicles. The dehydration-rehydration technique was adjusted to retain more than 60% of the plasmids in the oleic acid-containing vesicles. Still, most of the retained plasmids were released by squeezing through 0.45 μm filters, indicating very large vesicles. Smaller vesicles prepared by detergent dialysis with oleic acid re-added to the lipid mixture had a 15% capture rate in 200 nm liposomes.
Figure 1. Transmission electron microscopic images of the pDNA-encapsulating cationic liposomes. (Obata et al., 2009).
Lifeasible can provide custom liposome-encapsulated plasmid DNA services. Liposomes encapsulating plasmid DNA (pDNA) are prepared by mixing an ethanolic solution of lipids with an aqueous solution of plasmid DNA. After the product is customized, we can also provide you with follow-up services, including liposome content, the zeta potential of liposomes, TEM testing of encapsulated pDNA liposomes, cellular uptake efficiency, fusion potential to biofilms, gene expression efficiency of encapsulated pDNA liposomes, and cytotoxicity of liposomes.
The liposome encapsulation technologies we offer are not limited to the above. In addition to the above liposome-encapsulated plasmid DNA services, Lifeasible can provide encapsulation services for other types of molecules. Our technical team is constantly working on liposome development services, and the types of services are constantly being updated, so keep an eye on us, and we will provide you with the services you need.
Lifeasible provides you with a service that meets market standards. We conduct quality control at every step of the liposome technology development process and rigorously test the final results to ensure that our stringent quality standards are met. By continuously developing and optimizing our liposome technology, we can ensure that the service you receive is of the highest quality.
If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.
Reference
