Liposomes are composite structures made of phospholipids that are artificially created to encapsulate various types of nucleic acids within the aqueous core of liposomes. Due to the hydrophobic nature of the liposome outer membrane, it can be fused to other lipid bilayers, such as cell membranes, and deliver its contents via cell fusion. Lifeasible offers encapsulation of siRNA molecules into several standard liposome formulations (PC: cholesterol and DSPC: cationic lipids: polyethylene glycolated lipids: cholesterol) or custom liposome formulations. Custom experimental designs of liposomes can be used to provide siRNA protection (in the liposome core) in PEG-modified liposomes, where biodegradable lipids and low-toxicity polymers are used in the preparation process.
siRNAs have sequence-specific targeting and their potential to inhibit protein translation. Liposomes composed of amphiphilic lipids, including phospholipids, can trap hydrophilic or hydrophobic materials in hydrophilic internal or hydrophobic lipid bilayers, respectively. Based on these properties, liposomes have been developed as delivery cargoes for various molecules, such as small molecules, nucleic acids (including siRNA and proteins), etc. Substances encapsulated in liposomes offer many advantages, such as preventing degradation and reducing substance toxicity.
Figure 1. Scheme of the siRNA/SML preparation. (Mokhtarieh et al., 2018)
Lifeasible offers liposome-encapsulated siRNA services, including liposome preparation, size customization, liposome characterization testing, analysis, and more.
Liposomes can be prepared by ultrasonication, liposome extrusion, and micro-fluidization methods, and liposome sizes can be customized from 50 nm to 400 nm.
In addition to custom wrapping services for siRNA, we can customize other types of nucleic acids or small molecules for our customers. Please contact our staff to find the right product solution for you if you have a need.
Customers should provide at least 0.25 mg of siRNA for liposome encapsulation.
Liposome-encapsulated siRNA is delivered to the customer in sterile vials to ensure consistency and eliminate potential contamination.
Lifeasible's experienced staff ensures high-quality liposome manufacturing processes and cost-effective, reliable, and consistently high-quality products. At Lifeasible, we are committed to excellence while providing quality-assured products for R&D. Lifeasible provides integrated services for developing and testing all liposomal products, helping customers prepare their liposomal products for the market.
If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.
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