Liposome Membrane Permeability Testing

Small liposome molecules need to pass through a series of biological membranes to function. Therefore, the prediction of membrane permeability of small molecules is very important. Liposomes can mimic the structure of natural biological membranes, and their good stability and cellular affinity are often used as quasi-stationary or stationary phase in capillary electrophoresis (CE) analysis. Liposome capillary electrophoresis, which uses liposome solution as the running medium, can be a simple, effective, and stable model for in vitro screening small molecules.

About Micellar Capillary Electrochromatography

Micellar capillary electrochromatography (MEKC) is a fast-developing and widely used electrokinetic chromatography technique, which is often used to simulate the hydrophobicity of compounds evaluated by biological membranes due to its hydrophobic and hydrophilic moieties. The separation principle is based on adding a quasi-stationary phase (commonly used surfactants, charged micelles, or microemulsions with their mobility) to a buffer solution and separating compounds according to their electrophoretic differences and differences in partitioning properties in the micelles. The MEKC method is often used as an alternative method to assess the lipophilicity of compounds due to its simplicity, rapidity of operation, and small sample size.

Our Services

Lifeasible uses liposome electrokinetic chromatography (LEKC), in which liposomes are added to buffers as quasi-mobilized phases, and immobilized liposome capillary column chromatography (ILCCC), in which liposomes are covalently immobilized on the inner walls of capillaries, to study the interaction between small molecules and mimic membranes and thus evaluate the membrane permeability of small molecules.

Advantages of liposome capillary electrophoresis

1. The bilayer phospholipid structure of liposomes is closer to the cellular biofilm structure, which better mimics the fluidity of the lipid bilayer of biological membranes.
2. Fast, simple, less sample volume required, less requirement for sample purity, strong separation ability, easy to automate.

Service Content

• Analysis of liposome membrane permeability, assessment
• Solutions for liposome permeability testing
• Raw and analytical data for testing

Service Process

• Contact us and request the test you need.
• Send your samples.
• Sign a contract and confidentiality agreement.
• Develop experimental protocol and conduct experiments.
• Provide data report.

Our Advantages

• Short experimental cycle time and fast content delivery.
• Good prices because we offer integrated services and reduce many intermediate processes.
• Perfect after-sales service; you can always consult us to get the information you want.
• Rich project experience, mature technical system, and professional, scientific staff.
• A complete set of experimental services, from the custom synthesis of samples, and various types of analysis to final data reporting.

If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.