Intracellular pH (pH) is an important physiological determinant of enzyme activity and cellular function, and all proteins depend on a tightly regulated pH to maintain their structure and function. Intracellular pH, as an essential regulator of cellular physiological activities, not only regulates enzyme activities and some critical metabolic processes, but also many physiological activities such as ATP synthesis, DNA replication, RNA and protein synthesis, and cell growth are regulated by intracellular pH. More and more experiments have shown that changes in intracellular pH act as a second messenger in physiological activities such as apical growth, root gravitropism, plant defense, and phytohormone response. The pH value in the cytoplasm of most plants is close to neutral, but there will be some changes under special physiological conditions or external environmental factors. The timely intracellular pH regulation of plant cells is an important feature of all living cells, and intracellular pH is also an important parameter in the current study of cell function. Therefore, accurate, dynamic, and non-destructive observation of intracellular pH is important for the in-depth analysis of the relationship between cell metabolism and intracellular pH and the related mechanism of action.
Lifeasible uses suitable methods for measuring intracellular pH according to different experimental materials before measurement. Currently, we mainly utilize nuclear magnetic resonance (NMR), microelectrode, and fluorescent dye methods to determine intracellular pH in plants.
In addition, we can also use the photoelectric method for the determination of intracellular pH in plant cells, using photodetectors to measure the photoelectric signals inside the cell and inferring the changes in intracellular pH through the changes in the signals, which is a more accurate method.
1. Experimental steps
2. Relevant parameters
3. Pictures
4. raw data
5. Information on plant pH content
Lifeasible has many years of experience in plant cellular measurement and functional analysis, and we always follow the development of advanced technology, using various advanced technology methods, which can determine plant pH value efficiently and accurately. In addition, we also provide a full range of other plant analysis services to meet your different needs. If you are interested in us, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following: