Based on advanced RNA-sequencing (RNA-Seq) technology and bioinformatics infrastructure, Lifeasible offers mushroom differential transcriptomics services in different environmental treatments to sequence mushrooms under blue light irradiation, darkness, and hormonal conditions to screen specifically expressed genes.
Introduction to Mushroom Transcriptomics in Different Environments
Mushrooms are susceptible to environmental factors such as hormones, light, and temperature during growth. For example, the metabolism of mushrooms is regulated by the use of light by mycelium and zygotes. These factors have a serious impact on the primitive development of mushrooms, the differentiation of substrates, and the coloration of mycelium after maturation. However, the underlying molecular mechanisms controlling these processes and the expression of hormone-, light-, and temperature-induced genes in mushrooms remain unclear. Researchers have used transcriptomics techniques to screen specifically expressed genes under different treatments and explore their stress response mechanisms to maintain normal growth and development of mushrooms in response to changes in the external environment.
Fig. 1. KOG functional analysis of 2-fold upregulated genes under blue light conditions. (Kim J Y, et al., 2020)
Services
The actual cultivation of mushrooms is susceptible to changes due to the external environment. Our experts are very interested in the underlying mechanisms that cause such changes. We perform transcriptomic analysis of mushrooms under different treatments and compare the transcriptomic data before and after treatment to screen for differentially expressed genes that may be involved in stress regulatory responses. Lifeasible provides professional mushroom transcriptomics services under different environmental treatments.
We offer transcriptomics techniques to analyze the effects of hormones on mushrooms. We perform transcriptome sequencing on hormone-sprayed and control mushrooms to screen for differentially expressed genes and enrich for metabolic pathways in which the genes are involved.
The metabolism of mushrooms is regulated by the use of light by mycelium and zygotes. We use transcriptomic techniques to sequence mushrooms under blue light irradiation and dark conditions to compare morphological changes in substrates and analyze gene expression.
Mushrooms respond to low-temperature stress by controlling the process of substance synthesis. We can sequence the transcriptome by extracting RNA from mushrooms and performing enrichment and quantitative expression analysis of differentially expressed genes, focusing on genes related to the substance transport pathway.
Mushroom cultivation encounters environments other than hormones, temperature, and light. We develop transcriptomic technologies to allow access to more differentially expressed genes and increase gene stocks.
Transcriptomics technology not only allows the screening a large number of functional genes for transgenic mushrooms but also provides some reliable cultivation measures for practical production. In addition to molecular-level studies such as functional validation of the screened differential genes, we also combined transcriptomics technology with the results of relevant physiological and biochemical indexes to try to jointly analyze the response mechanism of mushrooms. Focusing on the transcriptome level and physiological level, we help our clients to develop appropriate cultivation measures to improve the mushroom's adaptability in production adversity. If you are interested in our services, please contact us.
Reference
- Kim J Y, et al. (2020) Transcriptome analysis of the edible mushroom Lentinula edodes in response to blue light[J]. PLoS One. 15(3): e0230680.
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