Inflammation is the natural protective response of the innate immune system to tissue injury or damaging external stimuli. Chronic inflammation increases the risk of various cancers. Mushroom metabolites have been found to be able to inhibit the production of inflammatory mediators and have received widespread attention.
Lifeasible provides one-stop preclinical research services for the anti-inflammatory drug development of mushroom metabolites. From the extraction and separation of anti-inflammatory metabolites, and in vitro mechanism research to preclinical animal research, we can meet your preclinical research needs for anti-inflammatory drug development.
Anti-Inflammatory Mechanisms of Mushrooms
Inflammation is a complex process mediated by cytokines, chemokines, adhesion molecules, and cytoplasmic nuclear receptors. Many mechanisms have been identified for the anti-inflammation activity of mushrooms, including inhibition of secretion of inflammatory cytokine, prevention of oxidation, regulation of the host immune system, and changes in cellular signaling. For example, Ergosterols from mushrooms suppress LPS-induced inflammatory responses through the inhibition of NF-κB and CCAAT/enhancer-binding protein beta transcriptional activity and phosphorylation of mitogen-activated protein kinases (MAPKs).
Fig. 1 Mushroom metabolites exert the anti-inflammation effect via the NF-κB pathway. (Du B, et al., 2018)
Our Services
Lifeasible provides research services for the development of anti-inflammatory drugs from mushroom metabolites. Our services include but are not limited to the following:
Isolation of active secondary metabolites from mushrooms
We will formulate extraction and separation schemes for target secondary metabolites according to your needs to speed up your experimental process.
Anti-inflammatory mechanism research services
- In vitro studies
- In vitro model construction service
We provide a variety of anti-inflammatory in vitro model construction services, such as lipopolysaccharide (LPS)-induced macrophages model, concanavalin A (ConA)-stimulated mouse splenocytes model, IFN-γ activated murine macrophages model, cyclooxygenase-1 and cyclooxygenase-2 enzymes model, etc.
- Inflammatory cytokine measurement service
We assessed the anti-inflammatory capacity of mushroom metabolites by multiplexed assays of cytokines such as TNF-α and IL-1β.
- Animal studies
We provide animal-level anti-inflammatory mechanism research services, construct animal models of inflammation, and reveal the anti-inflammatory mechanism of mushroom secondary metabolites. Since we can provide many animal models, the following models are for reference only. If you have other requirements for inflammation animal model construction, please contact us for detailed consultation.
- Dextran sulfate sodium (DSS)-induced colitis model
- Carrageenan and dextran-induced acute inflammatory mice models
- Formalin-induced chronic inflammatory mice models
- Phorbol ester-induced mouse skin model
- Acetic acid-induced mice model
- Nystatin-induced rat paw edema
- Freund’s adjuvant-induced arthritis
- Stress-induced gastric ulcers model
- Egg-white induced rat paw edema model
Advantages of Our Services
- Cytokines do not operate independently but in a network of activation and inhibition. Assessing the inflammatory response by detecting a single cytokine often cannot reflect the complexity of inflammation. To overcome this problem, our multiplex or multifactor assay is very efficient because it captures many different secreted cytokines, which allows us to analyze hundreds of targets simultaneously.
- Perfect platform for in vitro and animal experiments.
- A variety of in vitro and animal models to meet different experimental needs.
Contact Us
Lifeasible provides one-stop preclinical research services for anti-inflammatory drug development of mushroom secondary metabolites. Promote drug development and application of mushroom secondary metabolites. If you are interested in our research services, please contact us for a more detailed description of our services.
Reference
- Du, B.; et al. An insight into the anti-inflammatory properties of edible and medicinal mushrooms. Journal of Functional Foods. 2018, 47: 334-342.
For research use only, not for clinical use.
