For thousands of years, edible mushrooms have been used as food and flavoring agents. However, the degradation of strains has limited the edible mushroom industry's development and reduced its biotechnological value. Volvariella volvacea is a typical eatable straw mushroom with high-temperature tolerance. Its substrates are popular with consumers for their pleasant taste and high nutritional content. At present, the straw mushroom is also facing problems with the degradation of strain viability and product quality during production.
Lifeasible provides tissue culture services for Volvariella volvacea. Develop varieties that produce high-quality seeds by breeding excellent parent strains of straw mushrooms using tissue culture. We are committed to supporting research that promotes the industrial production of economically significant edible mushrooms.
What is Volvariella volvacea?
Volvariella volvacea (also known as paddy straw mushroom or straw mushroom) is a species of edible mushroom cultivated throughout East and Southeast Asia. Straw mushroom mycelium is colorless and transparent, with cells of varying lengths, 46-400 µm in diameter, averaging 217 µm, 6-18 µm wide, averaging 10 µm, separated by septa into a multicellular mycelium that branches and spreads constantly, intertwining to form a loose mesh-like mycelium. Volvariella volvacea is a nutritious mushroom widely grown in many parts of Asia using agricultural waste (rice straw, cotton waste) as a growing substrate.
Fig. 1 Life cycle and the fruiting body of Volvariella volvacea. (Bao D, et al., 2013)
Our Services
Lifeasible offers a service to study the cultivation of straw mushrooms from tissue culture and to monitor the effect of pure culture on strain quality and mushroom production. The highest radial growth rates can be achieved in Petri dishes for mushrooms grown using our tissue culture technique. We also offer several types of mushroom tissue culture services and can provide tissue culture solutions for specific strains according to your experimental needs.
Service Workflow
- Selection of the largest egg-stage straw mushroom substrates
- Surface disinfection with 70% ethanol
- Rinse 2-3 times with sterile water
- Halve cotyledons lengthwise
- Transfer small pieces of tissue from the stem and gill joints to sterilized PDA plates
- Transfer 5 pieces of tissue onto 5 slopes, starting from the mono-oval stage
- Select pure cultures from the tissue culture
Contact Us
Lifeasible provides tissue culture services for Volvariella volvacea. If you are interested in our services, please contact us for a more detailed description of our services.
References
- Swagatika B, et al. Effect of source of pure culture of Volvariella volvacea (bull. fries) singer on its growth and yield. Curr.Microbiol. 2021; 10(5): 738-743.
- Bao D, et al. Sequencing and comparative analysis of the straw mushroom (Volvariella volvacea) genome. PLoS One. 2013; 8 (3): e58294.
For research use only, not for clinical use.
