Nano Antibody Screening

Screening for highly specific antibodies is often required in disease treatment, diagnostic reagents, and protein structure research. Suppose homologous proteins of the same family are relatively homologous to each other. In that case, it can pose a great challenge for antibody development, and the type of preparation technology and screening method used is crucial. Yeast surface display is a eukaryotic protein expression system, a class of high-throughput screening methods for screening protein mutant libraries and is widely used in studies such as antibody expression, in vitro maturation, and screening with high efficiency and sensitivity.

Compared with the traditional phage display technology, the yeast surface display system is a new method that combines random mutagenesis and combinatorial libraries to screen peptides and proteins with specific properties. It can avoid the non-specific binding of phage itself to target proteins and is an important complement to the phage surface display system.

In the process of obtaining nanobodies using the yeast display system, Lifeasible displays the nanobodies on the yeast surface. After sorting positive clones by FACS, diverse antibody sequences can be obtained by sequencing. Our service provides an excellent mimicry of the structure of all nanobodies on the cell membrane surface, correctly reflecting the binding of nanobodies to antigens with very little non-specific binding common to phage display libraries. In addition, the yeast display library technique is also more cost-effective when performing multiple nanobody screens.

Technical advantages

• Antibody library yeast surface display has significant advantages over phage display. Yeast has a secretion pathway similar to that of higher eukaryotes. Proteins are folded in the endoplasmic reticulum, where chaperone proteins, folding enzymes, and quality control mechanisms ensure that only correctly folded proteins are secreted.
• Screening for higher affinity antibodies by phage display is usually adversely affected by the screening process, depending not only on affinity but also on antibody expression level. In contrast, we used FACS sorting technology to screen based on antibody affinity and display levels, thus eliminating expression-induced bias and distinguishing between clones with minimal differences in affinity.
• By using double-stained FACS, antibody affinity can be determined directly on the yeast cell surface, thus eliminating the need for time-consuming subcloning, expression, and purification.
• Antibody screening is fast, and the affinity and stability of the antibody can be optimized under the same display system.

Yeast surface display technology is a versatile set of techniques applied in antibody discovery, target discovery, and protein engineering. In antibody engineering, Lifeasible takes advantage of the properties of the yeast eukaryotic expression system to express antibody library proteins on the surface of yeast cells, which are screened using magnetic beads and flow cell sorting techniques to obtain high-affinity or high-stability antibody sequences. Please contact our staff for more information.