Natural products are secondary metabolites produced by plants, microorganisms, and other organisms during long-term evolution, and they are of great value in medicine, agriculture, and food. However, traditional natural product extraction methods have problems such as low efficiency, high cost, and high environmental pressure, and many high-value natural products have extremely low content in the host, and the synthesis pathways are complex, making it difficult to produce them on a large scale.

With the development of synthetic biology and histology technology, it has become a core direction to break through the traditional bottleneck by analyzing the biosynthetic pathways of natural products and utilizing engineered chassis cells for large-scale preparation. Lifeasible is a cutting-edge agro-biological company that combines agricultural biosynthesis with agricultural resources and synthetic biotechnology; to develop highly efficient and sustainable natural product production systems we aim to develop efficient and sustainable natural product production systems that promote the convergence of agriculture and biomanufacturing.

Significance of Natural Product Synthesis Pathway Analysis and Scale-up Preparation

• Analyzing the biosynthetic pathways of natural products helps to understand various enzymatic reactions and gene regulatory mechanisms in plants or microorganisms, providing essential clues for basic biological research.
• After clarifying the key enzymes and regulatory nodes, genetic engineering and metabolic engineering can optimize the synthetic pathway and significantly increase the accumulation of the target products in microorganisms or plants, thus achieving efficient preparation.
• Replacing traditional chemical synthesis by biosynthesis method can reduce energy consumption and raw material consumption, and avoid using toxic chemical reagents, which is in line with the requirements of green production and sustainable development.

Our Service for Natural Product Synthesis Pathway Analysis and Scale-up Preparation

Natural product synthesis pathway analysis technology

• Chemical proteomics. Labeling of key enzymes with active molecular probes, combined with mass spectrometry to identify functional proteins in the synthetic pathway.
• Multi-omics integration analysis. Combined genomic, transcriptomic, and metabolomic analysis to mine candidate genes and regulatory networks.
• Gene editing technology. Knockout or overexpression of target genes to validate pathway function using gene editing techniques.

Using multi-omics data and bioinformatics analysis, we can precisely reconstruct the synthesis network of the target natural products in the organism. The specific steps are shown below.

Gene and enzyme screening and validation. Identify key catalytic enzymes and regulatory factors through high-throughput sequencing and data mining.

Metabolic network construction and simulation. Simulate, predict, and optimize the whole metabolic pathway with the help of computational models and system biology tools to ensure the rationality and efficiency of the synthetic pathway design.

Genetic engineering and strain construction

• Aiming at the key nodes analyzed, we adopt gene editing and synthetic biology methods to construct modified strains in target microbes or plant hosts. Through pathway reconstruction and metabolic regulation, we optimize the expression and activity of key enzymes, thus improving the accumulation of target products.

Scale-up preparation technology

• Metabolic engineering modification. Optimize metabolic flow in chassis cells (e.g., yeast, E. coli) to enhance product accumulation efficiency.
• Fermentation process optimization. Achieve industrial production through reactor design and media optimization.
• Separation and purification platform. High-efficiency chromatography technology (e.g., HPLC) and membrane separation technology to ensure product purity.

Our Service Process

• Demand analysis and target confirmation. Clarify the customer's demand for the target product (e.g., a specific active ingredient) and yield requirements.
• Synthetic pathway analysis. Mining candidate genes through multi-omics data and utilizing gene editing to verify function. We use chemical probes to label key enzymes and molecular docking simulation to analyze the catalytic mechanism.
• Component optimization and chassis construction. Design synthetic gene clusters and optimize promoters and regulatory elements to enhance expression efficiency. We also select suitable chassis (microorganisms/plant cells) to introduce the synthetic pathway.
• Scale-up and process development. We first optimize the laboratory's fermentation parameters, then scale up to production scale. It can also be combined with separation and purification technology to realize high-purity extraction.
• Quality control and standardization. We have established an analytical testing system and ensure our products are strictly standardized.

Fig.2 Our process of natural product synthesis pathway analysis and scale-up preparation. (Lifeasible)

Highlights of Our Natural Product Synthesis Pathway Analysis and Scale-up Preparation

• Technology integration capability. We have a whole chain of technology platforms for multi-omics analysis, gene editing, and metabolic engineering, which can rapidly resolve complex synthetic pathways.
• Full-process service coverage. We provide one-stop solutions from gene mining to industrialized production, reducing customers' R&D cycle and cost.
• Industry-academia-research cooperation. We build innovation carriers with universities, research institutes, and enterprises to share cutting-edge technology resources.
• Quality control system. We are certified by relevant regulatory agencies and equipped with advanced testing equipment (e.g., NMR, MS) to ensure product safety and stability.
• Green Sustainability. We adopt biosynthesis instead of chemical synthesis to reduce environmental pollution, and our services are more green and environmentally friendly.

The application of agricultural biosynthesis in the field of natural products will accelerate the transformation from laboratory to industry. Lifeasible is an industry leader in agricultural synthesis. As a service company, we will continue to focus on technological iteration and industry needs and are expected to occupy a leading position in agriculture, food, and so on. If you are interested, please feel free to contact us.