Oncorhynchus mykiss Gene Editing

Oncorhynchus mykiss, also known as rainbow trout because of its red stripes on the side of its body, belongs to the salmon family, Pacific salmon genus, and is rich in DHA, EPA, and vitamin B. It is an economically valuable food fish. The meat of rainbow trout is rich in unsaturated fatty acids, amino acids, and trace elements, which are beneficial to human absorption and nutritional balance and is very popular in Europe and America.

Figure 1. The rainbow trout (Oncorhynchus mykiss) is a trout native to cold-water tributaries of the Pacific Ocean in Asia and North America.

Rainbow trout are farmed on a large scale, but the effects of fish diseases and their slow growth rate have led to unmet market demand for rainbow trout. Using genome sequence and molecular biotechnology to target and edit rainbow trout genes to breed new species with high resistance and high growth rate is currently a hot spot in rainbow trout biology research and commercial selection.

As a world-renowned provider of fish gene editing services, Lifeasible has a mature and well-established CRISPR/Cas9 technology platform to provide you with one-stop Oncorhynchus mykiss gene editing services.

CRISPR/Cas9 Technology Services

CRISPR/Cas9 technology enables rapid and accurate targeted mutagenesis within the genome of rainbow trout cells. Among them, CRISPR/Cas ribonucleoprotein (RNP) complex technology mainly uses Cas9 protein and crRNA:tracrRNA double-stranded bodies to form RNP complexes, which are then delivered into rainbow trout cells or fertilized eggs by electroporation or microinjection to complete gene editing. This technique has great potential for cell line editing.

Lifeasible can provide a wide range of gene editing services for rainbow trout, including but not limited to the IGFBP-2b gene, dnd, OmA20, and lepra. The results of our experiments are genotyped and identified by Sanger sequencing, with extremely high editing efficiency and mutation rates.

Figure 2. Overview of the CRISPR/Cas9 gene editing strategy workflow in rainbow trout cells. (Zoppo, M, et al. 2021)

Key Genes for Gene Editing

• The product of the IGFBP gene usually binds to insulin-like growth factor (IGF) and affects the binding of ligands and surface receptors involved in the regulation of muscle and bone anabolism in rainbow trout.
• The dnd is involved in maintaining the function of germ cells, and its deletion in rainbow trout scale farming results in sterile offspring, ensuring that artificially edited rainbow trout genes do not flow into wild populations.
• The OmA20 in the Toll-like receptor (TLR) pathway is effective in suppressing NF-κB transcription factors and improving the resistance of rainbow trout to inflammation, especially that caused by LPS.
• The lepra plays a vital role in regulating appetite and participating in lipid metabolism in rainbow trout. The LepR mutation can produce a heavy feeding phenotype, resulting in large body weight, faster growth rate, and high visceral index rainbow trout.

Lifeasible can rapidly and efficiently edit rainbow trout cell lines for various gene deletions and insertions through CRISPR/Cas9 technology. It can also give scientists new ideas to study gene function and life activity mechanisms. Our CRISPR/Cas9 technology has significant advantages in terms of cost and time compared to traditional technologies. If you would like to learn more about our technical solutions, please contact us.