Product Name
CBP-tag | Calmodulin Binding Protein Eptiope Tag (monoclonal)
Immunogen
CBP epitope tag, sequence: KRRWKKNFIAVSAANRFKKISSSGAL
Background
CBP tag comes from muscle myosin light-chain kinase and contains 26 amino acid residues with the molecular weight of 4 kDa. This tag is characterized by the relatively high affinity for calmodulin (CaM), which makes it possible to purify CBP-tagged proteins from crude cell extracts using of a resin with CaM affinity. This antibody allows detection of CBP-tagged proteins.
Purity
Affinity purified in PBS pH 7.4. Contains 0.02 % sodium azide. Contains 50 % glycerol.
Reactivity
CBP-tagged proteins
Confirmed reactivity
Myc epitope tag
Expected/apparent MW
Depends upon protein fusion partner
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1: 500 - 1: 5000 (WB)
Storage
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.