BirA (mutated/TurboID) - HRP conjugated 40 µg

Name: BirA (mutated/TurboID) - HRP conjugated 40 µg
SKU: APA-AT-014

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Product Name

Cat#

APA-AT-014

Host

Rabbit

Immunogen

Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).

Background

Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.

Format

Liquid

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.

Quantity

40 µg

Reactivity

E. coli BirA - mutated/TurboID overexpressed in various organisms

Confirmed reactivity

BirA (mutated/TurboID)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Expected/apparent MW

Depends upon fusion partner

Recommended dilution

1 : 5000 (WB)

Storage

Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Datasheet

For research or industrial raw materials, not for personal medical use!

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