Product Name
MBP | Maltose-binding protein (monoclonal, clone 16D3)
Immunogen
MBP epitope tag protein.
Background
MBP (Maltose binding protein) is encoded by the malE gene of E.coli and is a commonly used tag when studying protein expression using a wide range of applications. MBP tag enables easy purification of proteins from bacterial extracts under mild conditions.
Purity
Purified on Protein A to a total immunoglobulin fraction, in Tris 0,1M, glycine 0,1M and 2 % sucrose.
Reactivity
MBP, Maltose-binding protein
Confirmed reactivity
MBP maltose binding protein epitope tag
Reconstitution
Reconstitute in distilled water.
Tested applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1:100 - 1:5 000 (ICC,IF), 1: 100 - 2 000 (WB)
Storage
Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.